The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation. / von Essen, Marina; Menné, Charlotte; Nielsen, Bodil L; Lauritsen, Jens Peter H; Dietrich, Jes; Andersen, Peter S; Karjalainen, Klaus; Ødum, Niels; Geisler, Carsten.

In: Journal of Immunology, Vol. 168, No. 9, 2002, p. 4519-23.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

von Essen, M, Menné, C, Nielsen, BL, Lauritsen, JPH, Dietrich, J, Andersen, PS, Karjalainen, K, Ødum, N & Geisler, C 2002, 'The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation', Journal of Immunology, vol. 168, no. 9, pp. 4519-23.

APA

von Essen, M., Menné, C., Nielsen, B. L., Lauritsen, J. P. H., Dietrich, J., Andersen, P. S., Karjalainen, K., Ødum, N., & Geisler, C. (2002). The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation. Journal of Immunology, 168(9), 4519-23.

Vancouver

von Essen M, Menné C, Nielsen BL, Lauritsen JPH, Dietrich J, Andersen PS et al. The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation. Journal of Immunology. 2002;168(9):4519-23.

Author

von Essen, Marina ; Menné, Charlotte ; Nielsen, Bodil L ; Lauritsen, Jens Peter H ; Dietrich, Jes ; Andersen, Peter S ; Karjalainen, Klaus ; Ødum, Niels ; Geisler, Carsten. / The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation. In: Journal of Immunology. 2002 ; Vol. 168, No. 9. pp. 4519-23.

Bibtex

@article{829260f0b0a011ddb538000ea68e967b,
title = "The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation",
abstract = "TCR down-regulation plays an important role in modulating T cell responses both during T cell development and in mature T cells. At least two distinct pathways exist for down-regulation of the TCR. One pathway is activated following TCR ligation and is dependent on tyrosine phosphorylation. The other pathway is dependent on protein kinase C (PKC)-mediated activation of the CD3 gamma di-leucine-based receptor-sorting motif. Previous studies have failed to demonstrate a connection between ligand- and PKC-induced TCR down-regulation. Thus, although an apparent paradox, the dogma has been that ligand- and PKC-induced TCR down-regulations are not interrelated. By analyses of a newly developed CD3 gamma-negative T cell variant, freshly isolated and PHA-activated PBMC, and a mouse T cell line, we challenged this dogma and demonstrate in this work that PKC activation and the CD3 gamma di-leucine-based motif are indeed required for efficient ligand-induced TCR down-regulation.",
author = "{von Essen}, Marina and Charlotte Menn{\'e} and Nielsen, {Bodil L} and Lauritsen, {Jens Peter H} and Jes Dietrich and Andersen, {Peter S} and Klaus Karjalainen and Niels {\O}dum and Carsten Geisler",
note = "Keywords: Amino Acid Motifs; Amino Acid Sequence; Animals; Antigens, CD3; Cell Line; Cells, Cultured; Down-Regulation; Gene Deletion; Humans; Jurkat Cells; Kinetics; Leucine; Ligands; Mice; Molecular Sequence Data; Protein Kinase C; Receptor-CD3 Complex, Antigen, T-Cell; Sequence Homology; T-Lymphocytes",
year = "2002",
language = "English",
volume = "168",
pages = "4519--23",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "9",

}

RIS

TY - JOUR

T1 - The CD3 gamma leucine-based receptor-sorting motif is required for efficient ligand-mediated TCR down-regulation

AU - von Essen, Marina

AU - Menné, Charlotte

AU - Nielsen, Bodil L

AU - Lauritsen, Jens Peter H

AU - Dietrich, Jes

AU - Andersen, Peter S

AU - Karjalainen, Klaus

AU - Ødum, Niels

AU - Geisler, Carsten

N1 - Keywords: Amino Acid Motifs; Amino Acid Sequence; Animals; Antigens, CD3; Cell Line; Cells, Cultured; Down-Regulation; Gene Deletion; Humans; Jurkat Cells; Kinetics; Leucine; Ligands; Mice; Molecular Sequence Data; Protein Kinase C; Receptor-CD3 Complex, Antigen, T-Cell; Sequence Homology; T-Lymphocytes

PY - 2002

Y1 - 2002

N2 - TCR down-regulation plays an important role in modulating T cell responses both during T cell development and in mature T cells. At least two distinct pathways exist for down-regulation of the TCR. One pathway is activated following TCR ligation and is dependent on tyrosine phosphorylation. The other pathway is dependent on protein kinase C (PKC)-mediated activation of the CD3 gamma di-leucine-based receptor-sorting motif. Previous studies have failed to demonstrate a connection between ligand- and PKC-induced TCR down-regulation. Thus, although an apparent paradox, the dogma has been that ligand- and PKC-induced TCR down-regulations are not interrelated. By analyses of a newly developed CD3 gamma-negative T cell variant, freshly isolated and PHA-activated PBMC, and a mouse T cell line, we challenged this dogma and demonstrate in this work that PKC activation and the CD3 gamma di-leucine-based motif are indeed required for efficient ligand-induced TCR down-regulation.

AB - TCR down-regulation plays an important role in modulating T cell responses both during T cell development and in mature T cells. At least two distinct pathways exist for down-regulation of the TCR. One pathway is activated following TCR ligation and is dependent on tyrosine phosphorylation. The other pathway is dependent on protein kinase C (PKC)-mediated activation of the CD3 gamma di-leucine-based receptor-sorting motif. Previous studies have failed to demonstrate a connection between ligand- and PKC-induced TCR down-regulation. Thus, although an apparent paradox, the dogma has been that ligand- and PKC-induced TCR down-regulations are not interrelated. By analyses of a newly developed CD3 gamma-negative T cell variant, freshly isolated and PHA-activated PBMC, and a mouse T cell line, we challenged this dogma and demonstrate in this work that PKC activation and the CD3 gamma di-leucine-based motif are indeed required for efficient ligand-induced TCR down-regulation.

M3 - Journal article

C2 - 11970997

VL - 168

SP - 4519

EP - 4523

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 9

ER -

ID: 8544579