T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens
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The present work demonstrates that antibody-induced cross-linking of MHC class I antigens on Jurkat T lymphoma cells leads to a rise in intracellular calcium (Cai2+) and, in the presence of phorbol ester (PMA), to IL-2 production and IL-2 receptor expression. The rise in Cai2+ exhibited a profile very different from that obtained after anti-CD3 antibody-induced activation suggesting that activation signals are transduced differently after binding of anti-CD3 antibody and class I cross-linking, respectively. However, when Cai2+ was examined in individual Jurkat cells by means of a digital image processing system no differences were observed after cross-linking with anti-CD3 and anti-MHC class I antibodies, respectively. Two CD3-negative mutant lymphoma lines were nearly totally refractory to class I cross-linking. Taken together our results may indicate the existence of a functional linkage between the T cell receptor complex and MHC class I molecules.
Original language | English |
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Journal | Cellular Immunology |
Volume | 126 |
Issue number | 1 |
Pages (from-to) | 196-210 |
Number of pages | 14 |
ISSN | 0008-8749 |
Publication status | Published - 1990 |
Bibliographical note
Keywords: Antigens, CD3; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Calcium; Histocompatibility Antigens Class I; Humans; Interleukin-2; Lymphocyte Activation; Receptors, Antigen, T-Cell; Receptors, Interleukin-2; Signal Transduction; T-Lymphocytes
ID: 8546392