T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens

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Standard

T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens. / Dissing, S; Geisler, C; Rubin, B; Plesner, T; Claesson, M H.

In: Cellular Immunology, Vol. 126, No. 1, 1990, p. 196-210.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Dissing, S, Geisler, C, Rubin, B, Plesner, T & Claesson, MH 1990, 'T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens', Cellular Immunology, vol. 126, no. 1, pp. 196-210.

APA

Dissing, S., Geisler, C., Rubin, B., Plesner, T., & Claesson, M. H. (1990). T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens. Cellular Immunology, 126(1), 196-210.

Vancouver

Dissing S, Geisler C, Rubin B, Plesner T, Claesson MH. T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens. Cellular Immunology. 1990;126(1):196-210.

Author

Dissing, S ; Geisler, C ; Rubin, B ; Plesner, T ; Claesson, M H. / T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens. In: Cellular Immunology. 1990 ; Vol. 126, No. 1. pp. 196-210.

Bibtex

@article{8ec1c200b0a811ddb538000ea68e967b,
title = "T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens",
abstract = "The present work demonstrates that antibody-induced cross-linking of MHC class I antigens on Jurkat T lymphoma cells leads to a rise in intracellular calcium (Cai2+) and, in the presence of phorbol ester (PMA), to IL-2 production and IL-2 receptor expression. The rise in Cai2+ exhibited a profile very different from that obtained after anti-CD3 antibody-induced activation suggesting that activation signals are transduced differently after binding of anti-CD3 antibody and class I cross-linking, respectively. However, when Cai2+ was examined in individual Jurkat cells by means of a digital image processing system no differences were observed after cross-linking with anti-CD3 and anti-MHC class I antibodies, respectively. Two CD3-negative mutant lymphoma lines were nearly totally refractory to class I cross-linking. Taken together our results may indicate the existence of a functional linkage between the T cell receptor complex and MHC class I molecules.",
author = "S Dissing and C Geisler and B Rubin and T Plesner and Claesson, {M H}",
note = "Keywords: Antigens, CD3; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Calcium; Histocompatibility Antigens Class I; Humans; Interleukin-2; Lymphocyte Activation; Receptors, Antigen, T-Cell; Receptors, Interleukin-2; Signal Transduction; T-Lymphocytes",
year = "1990",
language = "English",
volume = "126",
pages = "196--210",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press",
number = "1",

}

RIS

TY - JOUR

T1 - T cell activation. II. Activation of human T lymphoma cells by cross-linking of their MHC class I antigens

AU - Dissing, S

AU - Geisler, C

AU - Rubin, B

AU - Plesner, T

AU - Claesson, M H

N1 - Keywords: Antigens, CD3; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Calcium; Histocompatibility Antigens Class I; Humans; Interleukin-2; Lymphocyte Activation; Receptors, Antigen, T-Cell; Receptors, Interleukin-2; Signal Transduction; T-Lymphocytes

PY - 1990

Y1 - 1990

N2 - The present work demonstrates that antibody-induced cross-linking of MHC class I antigens on Jurkat T lymphoma cells leads to a rise in intracellular calcium (Cai2+) and, in the presence of phorbol ester (PMA), to IL-2 production and IL-2 receptor expression. The rise in Cai2+ exhibited a profile very different from that obtained after anti-CD3 antibody-induced activation suggesting that activation signals are transduced differently after binding of anti-CD3 antibody and class I cross-linking, respectively. However, when Cai2+ was examined in individual Jurkat cells by means of a digital image processing system no differences were observed after cross-linking with anti-CD3 and anti-MHC class I antibodies, respectively. Two CD3-negative mutant lymphoma lines were nearly totally refractory to class I cross-linking. Taken together our results may indicate the existence of a functional linkage between the T cell receptor complex and MHC class I molecules.

AB - The present work demonstrates that antibody-induced cross-linking of MHC class I antigens on Jurkat T lymphoma cells leads to a rise in intracellular calcium (Cai2+) and, in the presence of phorbol ester (PMA), to IL-2 production and IL-2 receptor expression. The rise in Cai2+ exhibited a profile very different from that obtained after anti-CD3 antibody-induced activation suggesting that activation signals are transduced differently after binding of anti-CD3 antibody and class I cross-linking, respectively. However, when Cai2+ was examined in individual Jurkat cells by means of a digital image processing system no differences were observed after cross-linking with anti-CD3 and anti-MHC class I antibodies, respectively. Two CD3-negative mutant lymphoma lines were nearly totally refractory to class I cross-linking. Taken together our results may indicate the existence of a functional linkage between the T cell receptor complex and MHC class I molecules.

M3 - Journal article

C2 - 2137376

VL - 126

SP - 196

EP - 210

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -

ID: 8546392