Cellular and molecular characteristics of transformed T cells from an antigen-specific T-cell line
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An antigen-specific T-cell line which transforms into T-lymphoma cells in vitro but apparently not in vivo is described. Membrane markers, tumorigenicity and T-cell receptor (TcR) V alpha and V beta-gene usage of the in vitro transformed T-cell line were analysed to investigate whether the transformation event was poly-, oligo-, or monoclonal. The results indicate that the T lymphoma has no chromosome abnormalities, contains no tumour-inducing virus, can induce clone-specific immunity, and is oligoclonal with respect to TcR V alpha and V beta expression. The nature of the transformation event and clinical application of vaccination against T lymphomas is discussed. In addition, the expressed TcR V alpha and V beta repertoire of Con A T blasts was apparently not affected by the Igh-l or the MHC haplotype, as investigated in Igh-l and MHC congeneic C57Bl mice.
Original language | English |
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Journal | Scandinavian Journal of Immunology |
Volume | 31 |
Issue number | 5 |
Pages (from-to) | 645-56 |
Number of pages | 11 |
ISSN | 0300-9475 |
Publication status | Published - 1990 |
Bibliographical note
Keywords: Animals; Antibodies, Monoclonal; Antigens, Neoplasm; Blotting, Northern; Blotting, Southern; Cell Line, Transformed; Cell Transformation, Neoplastic; DNA Probes; DNA, Neoplasm; Epitopes; Immunoglobulin Variable Region; Mice; Mice, Inbred Strains; Neoplasm Transplantation; Phenotype; RNA, Neoplasm; Receptors, Antigen, T-Cell; T-Lymphocytes
ID: 8546363