MicroRNA-155 is dysregulated in the skin of patients with vitiligo and inhibits melanogenesis-associated genes in melanocytes and keratinocytes
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Little is known about the functions of microRNAs (miRNAs) in skin pigmentation disorders. The aim of this study was to investigate the expression and potential role of miRNAs in vitiligo. Of 12 studied miRNAs with proven functions in cell proliferation, differentiation, immune responses and melanogenesis, miR-99b, miR-125b, miR-155 and miR-199a-3p were found to be increased and miR-145 was found to be decreased in the skin of patients with vitiligo. Combined pathway and target analysis revealed melanogenesis-associated targets for miR-99b, miR-125b, miR-155 and miR-199a-3p. In situ hybridization analysis demonstrated increased expression of miR-155 in the epidermis of patients with vitiligo. Correspondingly, miR-155 was induced by vitiligoassociated cytokines in human primary melanocytes and keratinocytes. When overexpressed, miR-155 inhibited the expression of melanogenesis-associated genes and altered interferon-regulated genes in melanocytes and keratinocytes. In conclusion, this study demonstrates that the expression of miRNAs is dysregulated in the skin of patients with vitiligo and suggests that miR-155 contributes to the pathogenesis of vitiligo.
Original language | English |
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Journal | Acta Dermato-Venereologica |
Volume | 96 |
Issue number | 6 |
Pages (from-to) | 742-747 |
Number of pages | 6 |
ISSN | 0001-5555 |
DOIs | |
Publication status | Published - Sep 2016 |
Bibliographical note
Funding Information:
This work was supported by personal research funds PUT177 and PUT214, an institutional research grant IUT2-2 from the Estonian Research Council, European Regional Fund with the Archimedes Foundation and EU structural assistance grants SARMP12219T and SARBS12096T.
Publisher Copyright:
© 2016 The Authors.
- Keratinocytes, Melanocytes, MiRNA, Skin, Vitiligo
Research areas
ID: 315464263