IL-2 and IL-15 regulate CD154 expression on activated CD4 T cells
Research output: Contribution to journal › Journal article › Research › peer-review
The cellular and humoral immune system is critically dependent upon CD40-CD154 (CD40 ligand) interactions between CD40 expressed on B cells, macrophages, and dendritic cells, and CD154 expressed primarily on CD4 T cells. Previous studies have shown that CD154 is transiently expressed on CD4 T cells after T cell receptor engagement in vitro. However, we found that stimulation of PBLs with maximal CD28 costimulation, using beads coupled to Abs against CD3 and CD28, led to a very prolonged expression of CD154 on CD4 cells (>4 days) that was dependent upon autocrine IL-2 production. Previously activated CD4 T cells could respond to IL-2, or the related cytokine IL-15, by de novo CD154 production and expression without requiring an additional signal from CD3 and CD28. These results provide evidence that CD28 costimulation of CD4 T cells, through autocrine IL-2 production, maintains high levels of CD154 expression. This has significant impact on our understanding of the acquired immune response and may provide insight concerning the mechanisms underlying several immunological diseases.
Original language | English |
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Journal | Journal of Immunology |
Volume | 164 |
Issue number | 7 |
Pages (from-to) | 3500-5 |
Number of pages | 5 |
ISSN | 0022-1767 |
Publication status | Published - 2000 |
Bibliographical note
Keywords: Antigen-Presenting Cells; Antigens, CD28; Antigens, CD3; Antigens, CD40; CD4-Positive T-Lymphocytes; CD40 Ligand; Calcineurin; Cell Communication; Cells, Cultured; Coculture Techniques; Humans; Interleukin-15; Interleukin-2; Ligands; Lymphocyte Activation; Membrane Glycoproteins; Time Factors; Tumor Cells, Cultured
ID: 10617376