IL-2 and IL-15 regulate CD154 expression on activated CD4 T cells

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Standard

IL-2 and IL-15 regulate CD154 expression on activated CD4 T cells. / Skov, S; Bonyhadi, M; Odum, Niels; Ledbetter, J A.

In: Journal of Immunology, Vol. 164, No. 7, 2000, p. 3500-5.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Skov, S, Bonyhadi, M, Odum, N & Ledbetter, JA 2000, 'IL-2 and IL-15 regulate CD154 expression on activated CD4 T cells', Journal of Immunology, vol. 164, no. 7, pp. 3500-5. <http://www.jimmunol.org/cgi/content/full/164/7/3500>

APA

Skov, S., Bonyhadi, M., Odum, N., & Ledbetter, J. A. (2000). IL-2 and IL-15 regulate CD154 expression on activated CD4 T cells. Journal of Immunology, 164(7), 3500-5. http://www.jimmunol.org/cgi/content/full/164/7/3500

Vancouver

Skov S, Bonyhadi M, Odum N, Ledbetter JA. IL-2 and IL-15 regulate CD154 expression on activated CD4 T cells. Journal of Immunology. 2000;164(7):3500-5.

Author

Skov, S ; Bonyhadi, M ; Odum, Niels ; Ledbetter, J A. / IL-2 and IL-15 regulate CD154 expression on activated CD4 T cells. In: Journal of Immunology. 2000 ; Vol. 164, No. 7. pp. 3500-5.

Bibtex

@article{ff4c1430fd0611ddb219000ea68e967b,
title = "IL-2 and IL-15 regulate CD154 expression on activated CD4 T cells",
abstract = "The cellular and humoral immune system is critically dependent upon CD40-CD154 (CD40 ligand) interactions between CD40 expressed on B cells, macrophages, and dendritic cells, and CD154 expressed primarily on CD4 T cells. Previous studies have shown that CD154 is transiently expressed on CD4 T cells after T cell receptor engagement in vitro. However, we found that stimulation of PBLs with maximal CD28 costimulation, using beads coupled to Abs against CD3 and CD28, led to a very prolonged expression of CD154 on CD4 cells (>4 days) that was dependent upon autocrine IL-2 production. Previously activated CD4 T cells could respond to IL-2, or the related cytokine IL-15, by de novo CD154 production and expression without requiring an additional signal from CD3 and CD28. These results provide evidence that CD28 costimulation of CD4 T cells, through autocrine IL-2 production, maintains high levels of CD154 expression. This has significant impact on our understanding of the acquired immune response and may provide insight concerning the mechanisms underlying several immunological diseases.",
author = "S Skov and M Bonyhadi and Niels Odum and Ledbetter, {J A}",
note = "Keywords: Antigen-Presenting Cells; Antigens, CD28; Antigens, CD3; Antigens, CD40; CD4-Positive T-Lymphocytes; CD40 Ligand; Calcineurin; Cell Communication; Cells, Cultured; Coculture Techniques; Humans; Interleukin-15; Interleukin-2; Ligands; Lymphocyte Activation; Membrane Glycoproteins; Time Factors; Tumor Cells, Cultured",
year = "2000",
language = "English",
volume = "164",
pages = "3500--5",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "7",

}

RIS

TY - JOUR

T1 - IL-2 and IL-15 regulate CD154 expression on activated CD4 T cells

AU - Skov, S

AU - Bonyhadi, M

AU - Odum, Niels

AU - Ledbetter, J A

N1 - Keywords: Antigen-Presenting Cells; Antigens, CD28; Antigens, CD3; Antigens, CD40; CD4-Positive T-Lymphocytes; CD40 Ligand; Calcineurin; Cell Communication; Cells, Cultured; Coculture Techniques; Humans; Interleukin-15; Interleukin-2; Ligands; Lymphocyte Activation; Membrane Glycoproteins; Time Factors; Tumor Cells, Cultured

PY - 2000

Y1 - 2000

N2 - The cellular and humoral immune system is critically dependent upon CD40-CD154 (CD40 ligand) interactions between CD40 expressed on B cells, macrophages, and dendritic cells, and CD154 expressed primarily on CD4 T cells. Previous studies have shown that CD154 is transiently expressed on CD4 T cells after T cell receptor engagement in vitro. However, we found that stimulation of PBLs with maximal CD28 costimulation, using beads coupled to Abs against CD3 and CD28, led to a very prolonged expression of CD154 on CD4 cells (>4 days) that was dependent upon autocrine IL-2 production. Previously activated CD4 T cells could respond to IL-2, or the related cytokine IL-15, by de novo CD154 production and expression without requiring an additional signal from CD3 and CD28. These results provide evidence that CD28 costimulation of CD4 T cells, through autocrine IL-2 production, maintains high levels of CD154 expression. This has significant impact on our understanding of the acquired immune response and may provide insight concerning the mechanisms underlying several immunological diseases.

AB - The cellular and humoral immune system is critically dependent upon CD40-CD154 (CD40 ligand) interactions between CD40 expressed on B cells, macrophages, and dendritic cells, and CD154 expressed primarily on CD4 T cells. Previous studies have shown that CD154 is transiently expressed on CD4 T cells after T cell receptor engagement in vitro. However, we found that stimulation of PBLs with maximal CD28 costimulation, using beads coupled to Abs against CD3 and CD28, led to a very prolonged expression of CD154 on CD4 cells (>4 days) that was dependent upon autocrine IL-2 production. Previously activated CD4 T cells could respond to IL-2, or the related cytokine IL-15, by de novo CD154 production and expression without requiring an additional signal from CD3 and CD28. These results provide evidence that CD28 costimulation of CD4 T cells, through autocrine IL-2 production, maintains high levels of CD154 expression. This has significant impact on our understanding of the acquired immune response and may provide insight concerning the mechanisms underlying several immunological diseases.

M3 - Journal article

C2 - 10725703

VL - 164

SP - 3500

EP - 3505

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 7

ER -

ID: 10617376