An enzyme-linked immunosorbent assay for autoantibodies against the nuclear protein Scl-70

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This paper describes the development of an enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of autoantibodies against the nuclear protein Scl-70. The isolation of Scl-70 from rat livers and the conditions for the ELISA are described. Compared with the already established double diffusion in gel for detection of anti-Scl-70 antibodies this ELISA has advantages.
Original languageEnglish
JournalJournal of Immunological Methods
Volume80
Issue number2
Pages (from-to)211-9
Number of pages8
ISSN0022-1759
Publication statusPublished - 1985

Bibliographical note

Keywords: Absorption; Animals; Antigens; Antigens, Nuclear; Autoantibodies; Autoantigens; Collodion; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Fluorescent Antibody Technique; Immunodiffusion; Lupus Erythematosus, Systemic; Nucleoproteins; Paper; Rats; Scleroderma, Systemic

ID: 8546782