A response calculus for immobilized T cell receptor ligands
Research output: Contribution to journal › Journal article › Research › peer-review
To address the molecular mechanism of T cell receptor (TCR) signaling, we have formulated a model for T cell activation, termed the 2D-affinity model, in which the density of TCR on the T cell surface, the density of ligand on the presenting surface, and their corresponding two-dimensional affinity determine the level of T cell activation. When fitted to T cell responses against purified ligands immobilized on plastic surfaces, the 2D-affinity model adequately simulated changes in cellular activation as a result of varying ligand affinity and ligand density. These observations further demonstrated the importance of receptor cross-linking density in determining TCR signaling. Moreover, it was found that the functional two-dimensional affinity of TCR ligands was affected by the chemical composition of the ligand-presenting surface. This makes it possible that cell-bound TCR ligands, despite their low affinity in solution, are of optimal two-dimensional affinity thereby allowing effective TCR binding under physiological conditions, i.e. at low ligand densities in cellular interfaces.
Original language | English |
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Journal | Journal of Biological Chemistry |
Volume | 276 |
Issue number | 52 |
Pages (from-to) | 49125-32 |
Number of pages | 7 |
ISSN | 0021-9258 |
DOIs | |
Publication status | Published - 2001 |
Bibliographical note
Keywords: Antibodies; Genes, Reporter; Ligands; Lymphocyte Activation; Mathematics; Models, Immunological; Protein Binding; Receptors, Antigen, T-Cell; Recombinant Fusion Proteins; Signal Transduction; Superantigens; T-Lymphocytes
ID: 8544701