MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue. / Yadav, Manoj Kumar; Ishida, Megumi; Gogoleva, Natalia; Liao, Ching Wei; Salim, Filiani Natalia; Kanai, Maho; Kuno, Akihiro; Hayashi, Takuto; Shahri, Zeynab Javanfekr; Kulathunga, Kaushalya; Samir, Omar; Lyu, Wenxin; Olivia, Olivia; Mbanefo, Evaristus C.; Takahashi, Satoru; Hamada, Michito.

In: Cell Reports, Vol. 43, No. 4, 113978, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Yadav, MK, Ishida, M, Gogoleva, N, Liao, CW, Salim, FN, Kanai, M, Kuno, A, Hayashi, T, Shahri, ZJ, Kulathunga, K, Samir, O, Lyu, W, Olivia, O, Mbanefo, EC, Takahashi, S & Hamada, M 2024, 'MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue', Cell Reports, vol. 43, no. 4, 113978. https://doi.org/10.1016/j.celrep.2024.113978

APA

Yadav, M. K., Ishida, M., Gogoleva, N., Liao, C. W., Salim, F. N., Kanai, M., Kuno, A., Hayashi, T., Shahri, Z. J., Kulathunga, K., Samir, O., Lyu, W., Olivia, O., Mbanefo, E. C., Takahashi, S., & Hamada, M. (2024). MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue. Cell Reports, 43(4), [113978]. https://doi.org/10.1016/j.celrep.2024.113978

Vancouver

Yadav MK, Ishida M, Gogoleva N, Liao CW, Salim FN, Kanai M et al. MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue. Cell Reports. 2024;43(4). 113978. https://doi.org/10.1016/j.celrep.2024.113978

Author

Yadav, Manoj Kumar ; Ishida, Megumi ; Gogoleva, Natalia ; Liao, Ching Wei ; Salim, Filiani Natalia ; Kanai, Maho ; Kuno, Akihiro ; Hayashi, Takuto ; Shahri, Zeynab Javanfekr ; Kulathunga, Kaushalya ; Samir, Omar ; Lyu, Wenxin ; Olivia, Olivia ; Mbanefo, Evaristus C. ; Takahashi, Satoru ; Hamada, Michito. / MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue. In: Cell Reports. 2024 ; Vol. 43, No. 4.

Bibtex

@article{6972aae225554bc6a245e731a3b230d0,
title = "MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue",
abstract = "Transcription factor MAFB regulates various homeostatic functions of macrophages. This study explores the role of MAFB in brown adipose tissue (BAT) thermogenesis using macrophage-specific Mafb-deficient (Mafbf/f::LysM-Cre) mice. We find that Mafb deficiency in macrophages reduces thermogenesis, energy expenditure, and sympathetic neuron (SN) density in BAT under cold conditions. This phenotype features a proinflammatory environment that is characterized by macrophage/granulocyte accumulation, increases in interleukin-6 (IL-6) production, and IL-6 trans-signaling, which lead to decreases in nerve growth factor (NGF) expression and reduction in SN density in BAT. We confirm MAFB regulation of IL-6 expression using luciferase readout driven by IL-6 promoter in RAW-264.7 macrophage cell lines. Immunohistochemistry shows clustered organization of NGF-producing cells in BAT, which are primarily TRPV1+ vascular smooth muscle cells, as additionally shown using single-cell RNA sequencing and RT-qPCR of the stromal vascular fraction. Treating Mafbf/f::LysM-Cre mice with anti-IL-6 receptor antibody rescues SN density, body temperature, and energy expenditure.",
keywords = "BAT, brown adipose tissue thermogenesis, CP: Immunology, CP: Metabolism, interleukin-6 (IL-6), MAFB, nerve growth factor, NGF, progenitors of brown adipocytes, SN, sympathetic neuronal density, TRPV1+ vascular smooth muscle cells",
author = "Yadav, {Manoj Kumar} and Megumi Ishida and Natalia Gogoleva and Liao, {Ching Wei} and Salim, {Filiani Natalia} and Maho Kanai and Akihiro Kuno and Takuto Hayashi and Shahri, {Zeynab Javanfekr} and Kaushalya Kulathunga and Omar Samir and Wenxin Lyu and Olivia Olivia and Mbanefo, {Evaristus C.} and Satoru Takahashi and Michito Hamada",
note = "Publisher Copyright: {\textcopyright} 2024 The Author(s)",
year = "2024",
doi = "10.1016/j.celrep.2024.113978",
language = "English",
volume = "43",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - MAFB in macrophages regulates cold-induced neuronal density in brown adipose tissue

AU - Yadav, Manoj Kumar

AU - Ishida, Megumi

AU - Gogoleva, Natalia

AU - Liao, Ching Wei

AU - Salim, Filiani Natalia

AU - Kanai, Maho

AU - Kuno, Akihiro

AU - Hayashi, Takuto

AU - Shahri, Zeynab Javanfekr

AU - Kulathunga, Kaushalya

AU - Samir, Omar

AU - Lyu, Wenxin

AU - Olivia, Olivia

AU - Mbanefo, Evaristus C.

AU - Takahashi, Satoru

AU - Hamada, Michito

N1 - Publisher Copyright: © 2024 The Author(s)

PY - 2024

Y1 - 2024

N2 - Transcription factor MAFB regulates various homeostatic functions of macrophages. This study explores the role of MAFB in brown adipose tissue (BAT) thermogenesis using macrophage-specific Mafb-deficient (Mafbf/f::LysM-Cre) mice. We find that Mafb deficiency in macrophages reduces thermogenesis, energy expenditure, and sympathetic neuron (SN) density in BAT under cold conditions. This phenotype features a proinflammatory environment that is characterized by macrophage/granulocyte accumulation, increases in interleukin-6 (IL-6) production, and IL-6 trans-signaling, which lead to decreases in nerve growth factor (NGF) expression and reduction in SN density in BAT. We confirm MAFB regulation of IL-6 expression using luciferase readout driven by IL-6 promoter in RAW-264.7 macrophage cell lines. Immunohistochemistry shows clustered organization of NGF-producing cells in BAT, which are primarily TRPV1+ vascular smooth muscle cells, as additionally shown using single-cell RNA sequencing and RT-qPCR of the stromal vascular fraction. Treating Mafbf/f::LysM-Cre mice with anti-IL-6 receptor antibody rescues SN density, body temperature, and energy expenditure.

AB - Transcription factor MAFB regulates various homeostatic functions of macrophages. This study explores the role of MAFB in brown adipose tissue (BAT) thermogenesis using macrophage-specific Mafb-deficient (Mafbf/f::LysM-Cre) mice. We find that Mafb deficiency in macrophages reduces thermogenesis, energy expenditure, and sympathetic neuron (SN) density in BAT under cold conditions. This phenotype features a proinflammatory environment that is characterized by macrophage/granulocyte accumulation, increases in interleukin-6 (IL-6) production, and IL-6 trans-signaling, which lead to decreases in nerve growth factor (NGF) expression and reduction in SN density in BAT. We confirm MAFB regulation of IL-6 expression using luciferase readout driven by IL-6 promoter in RAW-264.7 macrophage cell lines. Immunohistochemistry shows clustered organization of NGF-producing cells in BAT, which are primarily TRPV1+ vascular smooth muscle cells, as additionally shown using single-cell RNA sequencing and RT-qPCR of the stromal vascular fraction. Treating Mafbf/f::LysM-Cre mice with anti-IL-6 receptor antibody rescues SN density, body temperature, and energy expenditure.

KW - BAT

KW - brown adipose tissue thermogenesis

KW - CP: Immunology

KW - CP: Metabolism

KW - interleukin-6 (IL-6)

KW - MAFB

KW - nerve growth factor

KW - NGF

KW - progenitors of brown adipocytes

KW - SN

KW - sympathetic neuronal density

KW - TRPV1+ vascular smooth muscle cells

U2 - 10.1016/j.celrep.2024.113978

DO - 10.1016/j.celrep.2024.113978

M3 - Journal article

C2 - 38522069

AN - SCOPUS:85188521757

VL - 43

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 4

M1 - 113978

ER -

ID: 388041709