Laboratory mice with a wild microbiota generate strong allergic immune responses

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Laboratory mice with a wild microbiota generate strong allergic immune responses. / Ma, Junjie; Urgard, Egon; Runge, Solveig; Classon, Cajsa H.; Mathä, Laura; Stark, Julian M.; Cheng, Liqin; Álvarez, Javiera A.; von Zedtwitz, Silvia; Baleviciute, Austeja; Hoyer, Sergio Martinez; Li, Muzhen; Gernand, Anne Marleen; Osbelt, Lisa; Bielecka, Agata Anna; Lesker, Till R.; Huang, Huey Jy; Vrtala, Susanne; Boon, Louis; Beyaert, Rudi; Adner, Mikael; Martinez Gonzalez, Itziar; Strowig, Till; Du, Juan; Nylén, Susanne; Rosshart, Stephan P.; Coquet, Jonathan M.

In: Science immunology, Vol. 8, No. 87, adf7702, 2023, p. 1-16.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ma, J, Urgard, E, Runge, S, Classon, CH, Mathä, L, Stark, JM, Cheng, L, Álvarez, JA, von Zedtwitz, S, Baleviciute, A, Hoyer, SM, Li, M, Gernand, AM, Osbelt, L, Bielecka, AA, Lesker, TR, Huang, HJ, Vrtala, S, Boon, L, Beyaert, R, Adner, M, Martinez Gonzalez, I, Strowig, T, Du, J, Nylén, S, Rosshart, SP & Coquet, JM 2023, 'Laboratory mice with a wild microbiota generate strong allergic immune responses', Science immunology, vol. 8, no. 87, adf7702, pp. 1-16. https://doi.org/10.1126/sciimmunol.adf7702

APA

Ma, J., Urgard, E., Runge, S., Classon, C. H., Mathä, L., Stark, J. M., Cheng, L., Álvarez, J. A., von Zedtwitz, S., Baleviciute, A., Hoyer, S. M., Li, M., Gernand, A. M., Osbelt, L., Bielecka, A. A., Lesker, T. R., Huang, H. J., Vrtala, S., Boon, L., ... Coquet, J. M. (2023). Laboratory mice with a wild microbiota generate strong allergic immune responses. Science immunology, 8(87), 1-16. [adf7702]. https://doi.org/10.1126/sciimmunol.adf7702

Vancouver

Ma J, Urgard E, Runge S, Classon CH, Mathä L, Stark JM et al. Laboratory mice with a wild microbiota generate strong allergic immune responses. Science immunology. 2023;8(87):1-16. adf7702. https://doi.org/10.1126/sciimmunol.adf7702

Author

Ma, Junjie ; Urgard, Egon ; Runge, Solveig ; Classon, Cajsa H. ; Mathä, Laura ; Stark, Julian M. ; Cheng, Liqin ; Álvarez, Javiera A. ; von Zedtwitz, Silvia ; Baleviciute, Austeja ; Hoyer, Sergio Martinez ; Li, Muzhen ; Gernand, Anne Marleen ; Osbelt, Lisa ; Bielecka, Agata Anna ; Lesker, Till R. ; Huang, Huey Jy ; Vrtala, Susanne ; Boon, Louis ; Beyaert, Rudi ; Adner, Mikael ; Martinez Gonzalez, Itziar ; Strowig, Till ; Du, Juan ; Nylén, Susanne ; Rosshart, Stephan P. ; Coquet, Jonathan M. / Laboratory mice with a wild microbiota generate strong allergic immune responses. In: Science immunology. 2023 ; Vol. 8, No. 87. pp. 1-16.

Bibtex

@article{fc471efdd36a43fab20ff755b7c93263,
title = "Laboratory mice with a wild microbiota generate strong allergic immune responses",
abstract = "Allergic disorders are caused by a combination of hereditary and environmental factors. The hygiene hypothesis postulates that early-life microbial exposures impede the development of subsequent allergic disease. Recently developed {"}wildling{"}mice are genetically identical to standard laboratory specific pathogen-free (SPF) mice but are housed under seminatural conditions and have rich microbial exposures from birth. Thus, by comparing conventional SPF mice with wildlings, we can uncouple the impact of lifelong microbial exposures from genetic factors on the allergic immune response. We found that wildlings developed larger populations of antigen-experienced T cells than conventional SPF mice, which included interleukin-10-producing CD4 T cells specific for commensal Lactobacilli strains and allergy-promoting T helper 2 (TH2) cells. In models of airway exposure to house dust mite (HDM), recombinant interleukin-33, or Alternaria alternata, wildlings developed strong allergic inflammation, characterized by eosinophil recruitment, goblet cell metaplasia, and antigen-specific immunoglobulin G1 (IgG1) and IgE responses. Wildlings developed robust de novo TH2 cell responses to incoming allergens, whereas preexisting TH2 cells could also be recruited into the allergic immune response in a cytokinedriven and TCR-independent fashion. Thus, wildling mice, which experience diverse and lifelong microbial exposures, were not protected from developing pathological allergic immune responses. Instead, wildlings mounted robust allergic responses to incoming allergens, shedding new light on the hygiene hypothesis. ",
author = "Junjie Ma and Egon Urgard and Solveig Runge and Classon, {Cajsa H.} and Laura Math{\"a} and Stark, {Julian M.} and Liqin Cheng and {\'A}lvarez, {Javiera A.} and {von Zedtwitz}, Silvia and Austeja Baleviciute and Hoyer, {Sergio Martinez} and Muzhen Li and Gernand, {Anne Marleen} and Lisa Osbelt and Bielecka, {Agata Anna} and Lesker, {Till R.} and Huang, {Huey Jy} and Susanne Vrtala and Louis Boon and Rudi Beyaert and Mikael Adner and {Martinez Gonzalez}, Itziar and Till Strowig and Juan Du and Susanne Nyl{\'e}n and Rosshart, {Stephan P.} and Coquet, {Jonathan M.}",
note = "Publisher Copyright: {\textcopyright} 2023 The Authors.",
year = "2023",
doi = "10.1126/sciimmunol.adf7702",
language = "English",
volume = "8",
pages = "1--16",
journal = "Advances in Immunology",
issn = "0065-2776",
publisher = "American Association for the Advancement of Science",
number = "87",

}

RIS

TY - JOUR

T1 - Laboratory mice with a wild microbiota generate strong allergic immune responses

AU - Ma, Junjie

AU - Urgard, Egon

AU - Runge, Solveig

AU - Classon, Cajsa H.

AU - Mathä, Laura

AU - Stark, Julian M.

AU - Cheng, Liqin

AU - Álvarez, Javiera A.

AU - von Zedtwitz, Silvia

AU - Baleviciute, Austeja

AU - Hoyer, Sergio Martinez

AU - Li, Muzhen

AU - Gernand, Anne Marleen

AU - Osbelt, Lisa

AU - Bielecka, Agata Anna

AU - Lesker, Till R.

AU - Huang, Huey Jy

AU - Vrtala, Susanne

AU - Boon, Louis

AU - Beyaert, Rudi

AU - Adner, Mikael

AU - Martinez Gonzalez, Itziar

AU - Strowig, Till

AU - Du, Juan

AU - Nylén, Susanne

AU - Rosshart, Stephan P.

AU - Coquet, Jonathan M.

N1 - Publisher Copyright: © 2023 The Authors.

PY - 2023

Y1 - 2023

N2 - Allergic disorders are caused by a combination of hereditary and environmental factors. The hygiene hypothesis postulates that early-life microbial exposures impede the development of subsequent allergic disease. Recently developed "wildling"mice are genetically identical to standard laboratory specific pathogen-free (SPF) mice but are housed under seminatural conditions and have rich microbial exposures from birth. Thus, by comparing conventional SPF mice with wildlings, we can uncouple the impact of lifelong microbial exposures from genetic factors on the allergic immune response. We found that wildlings developed larger populations of antigen-experienced T cells than conventional SPF mice, which included interleukin-10-producing CD4 T cells specific for commensal Lactobacilli strains and allergy-promoting T helper 2 (TH2) cells. In models of airway exposure to house dust mite (HDM), recombinant interleukin-33, or Alternaria alternata, wildlings developed strong allergic inflammation, characterized by eosinophil recruitment, goblet cell metaplasia, and antigen-specific immunoglobulin G1 (IgG1) and IgE responses. Wildlings developed robust de novo TH2 cell responses to incoming allergens, whereas preexisting TH2 cells could also be recruited into the allergic immune response in a cytokinedriven and TCR-independent fashion. Thus, wildling mice, which experience diverse and lifelong microbial exposures, were not protected from developing pathological allergic immune responses. Instead, wildlings mounted robust allergic responses to incoming allergens, shedding new light on the hygiene hypothesis.

AB - Allergic disorders are caused by a combination of hereditary and environmental factors. The hygiene hypothesis postulates that early-life microbial exposures impede the development of subsequent allergic disease. Recently developed "wildling"mice are genetically identical to standard laboratory specific pathogen-free (SPF) mice but are housed under seminatural conditions and have rich microbial exposures from birth. Thus, by comparing conventional SPF mice with wildlings, we can uncouple the impact of lifelong microbial exposures from genetic factors on the allergic immune response. We found that wildlings developed larger populations of antigen-experienced T cells than conventional SPF mice, which included interleukin-10-producing CD4 T cells specific for commensal Lactobacilli strains and allergy-promoting T helper 2 (TH2) cells. In models of airway exposure to house dust mite (HDM), recombinant interleukin-33, or Alternaria alternata, wildlings developed strong allergic inflammation, characterized by eosinophil recruitment, goblet cell metaplasia, and antigen-specific immunoglobulin G1 (IgG1) and IgE responses. Wildlings developed robust de novo TH2 cell responses to incoming allergens, whereas preexisting TH2 cells could also be recruited into the allergic immune response in a cytokinedriven and TCR-independent fashion. Thus, wildling mice, which experience diverse and lifelong microbial exposures, were not protected from developing pathological allergic immune responses. Instead, wildlings mounted robust allergic responses to incoming allergens, shedding new light on the hygiene hypothesis.

UR - http://www.scopus.com/inward/record.url?scp=85172825484&partnerID=8YFLogxK

U2 - 10.1126/sciimmunol.adf7702

DO - 10.1126/sciimmunol.adf7702

M3 - Journal article

C2 - 37774008

AN - SCOPUS:85172825484

VL - 8

SP - 1

EP - 16

JO - Advances in Immunology

JF - Advances in Immunology

SN - 0065-2776

IS - 87

M1 - adf7702

ER -

ID: 373880511