Glycoengineered keratinocyte library reveals essential functions of specific glycans for all stages of HSV-1 infection

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Glycoengineered keratinocyte library reveals essential functions of specific glycans for all stages of HSV-1 infection. / Bagdonaite, Ieva; Marinova, Irina N.; Rudjord-Levann, Asha M.; Pallesen, Emil M. H.; King-Smith, Sarah L.; Karlsson, Richard; Rømer, Troels B.; Chen, Yen-Hsi; Miller, Rebecca L.; Olofsson, Sigvard; Nordén, Rickard; Bergström, Tomas; Dabelsteen, Sally; Wandall, Hans H.

In: Nature Communications, Vol. 14, 7000, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Bagdonaite, I, Marinova, IN, Rudjord-Levann, AM, Pallesen, EMH, King-Smith, SL, Karlsson, R, Rømer, TB, Chen, Y-H, Miller, RL, Olofsson, S, Nordén, R, Bergström, T, Dabelsteen, S & Wandall, HH 2023, 'Glycoengineered keratinocyte library reveals essential functions of specific glycans for all stages of HSV-1 infection', Nature Communications, vol. 14, 7000. https://doi.org/10.1038/s41467-023-42669-6

APA

Bagdonaite, I., Marinova, I. N., Rudjord-Levann, A. M., Pallesen, E. M. H., King-Smith, S. L., Karlsson, R., Rømer, T. B., Chen, Y-H., Miller, R. L., Olofsson, S., Nordén, R., Bergström, T., Dabelsteen, S., & Wandall, H. H. (2023). Glycoengineered keratinocyte library reveals essential functions of specific glycans for all stages of HSV-1 infection. Nature Communications, 14, [7000]. https://doi.org/10.1038/s41467-023-42669-6

Vancouver

Bagdonaite I, Marinova IN, Rudjord-Levann AM, Pallesen EMH, King-Smith SL, Karlsson R et al. Glycoengineered keratinocyte library reveals essential functions of specific glycans for all stages of HSV-1 infection. Nature Communications. 2023;14. 7000. https://doi.org/10.1038/s41467-023-42669-6

Author

Bagdonaite, Ieva ; Marinova, Irina N. ; Rudjord-Levann, Asha M. ; Pallesen, Emil M. H. ; King-Smith, Sarah L. ; Karlsson, Richard ; Rømer, Troels B. ; Chen, Yen-Hsi ; Miller, Rebecca L. ; Olofsson, Sigvard ; Nordén, Rickard ; Bergström, Tomas ; Dabelsteen, Sally ; Wandall, Hans H. / Glycoengineered keratinocyte library reveals essential functions of specific glycans for all stages of HSV-1 infection. In: Nature Communications. 2023 ; Vol. 14.

Bibtex

@article{486e57d306884968994a3407494cce07,
title = "Glycoengineered keratinocyte library reveals essential functions of specific glycans for all stages of HSV-1 infection",
abstract = "Viral and host glycans represent an understudied aspect of host-pathogen interactions, despite potential implications for treatment of viral infections. This is due to lack of easily accessible tools for analyzing glycan function in a meaningful context. Here we generate a glycoengineered keratinocyte library delineating human glycosylation pathways to uncover roles of specific glycans at different stages of herpes simplex virus type 1 (HSV-1) infectious cycle. We show the importance of cellular glycosaminoglycans and glycosphingolipids for HSV-1 attachment, N-glycans for entry and spread, and O-glycans for propagation. While altered virion surface structures have minimal effects on the early interactions with wild type cells, mutation of specific O-glycosylation sites affects glycoprotein surface expression and function. In conclusion, the data demonstrates the importance of specific glycans in a clinically relevant human model of HSV-1 infection and highlights the utility of genetic engineering to elucidate the roles of specific viral and cellular carbohydrate structures.",
author = "Ieva Bagdonaite and Marinova, {Irina N.} and Rudjord-Levann, {Asha M.} and Pallesen, {Emil M. H.} and King-Smith, {Sarah L.} and Richard Karlsson and R{\o}mer, {Troels B.} and Yen-Hsi Chen and Miller, {Rebecca L.} and Sigvard Olofsson and Rickard Nord{\'e}n and Tomas Bergstr{\"o}m and Sally Dabelsteen and Wandall, {Hans H.}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",
year = "2023",
doi = "10.1038/s41467-023-42669-6",
language = "English",
volume = "14",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Glycoengineered keratinocyte library reveals essential functions of specific glycans for all stages of HSV-1 infection

AU - Bagdonaite, Ieva

AU - Marinova, Irina N.

AU - Rudjord-Levann, Asha M.

AU - Pallesen, Emil M. H.

AU - King-Smith, Sarah L.

AU - Karlsson, Richard

AU - Rømer, Troels B.

AU - Chen, Yen-Hsi

AU - Miller, Rebecca L.

AU - Olofsson, Sigvard

AU - Nordén, Rickard

AU - Bergström, Tomas

AU - Dabelsteen, Sally

AU - Wandall, Hans H.

N1 - Publisher Copyright: © 2023, The Author(s).

PY - 2023

Y1 - 2023

N2 - Viral and host glycans represent an understudied aspect of host-pathogen interactions, despite potential implications for treatment of viral infections. This is due to lack of easily accessible tools for analyzing glycan function in a meaningful context. Here we generate a glycoengineered keratinocyte library delineating human glycosylation pathways to uncover roles of specific glycans at different stages of herpes simplex virus type 1 (HSV-1) infectious cycle. We show the importance of cellular glycosaminoglycans and glycosphingolipids for HSV-1 attachment, N-glycans for entry and spread, and O-glycans for propagation. While altered virion surface structures have minimal effects on the early interactions with wild type cells, mutation of specific O-glycosylation sites affects glycoprotein surface expression and function. In conclusion, the data demonstrates the importance of specific glycans in a clinically relevant human model of HSV-1 infection and highlights the utility of genetic engineering to elucidate the roles of specific viral and cellular carbohydrate structures.

AB - Viral and host glycans represent an understudied aspect of host-pathogen interactions, despite potential implications for treatment of viral infections. This is due to lack of easily accessible tools for analyzing glycan function in a meaningful context. Here we generate a glycoengineered keratinocyte library delineating human glycosylation pathways to uncover roles of specific glycans at different stages of herpes simplex virus type 1 (HSV-1) infectious cycle. We show the importance of cellular glycosaminoglycans and glycosphingolipids for HSV-1 attachment, N-glycans for entry and spread, and O-glycans for propagation. While altered virion surface structures have minimal effects on the early interactions with wild type cells, mutation of specific O-glycosylation sites affects glycoprotein surface expression and function. In conclusion, the data demonstrates the importance of specific glycans in a clinically relevant human model of HSV-1 infection and highlights the utility of genetic engineering to elucidate the roles of specific viral and cellular carbohydrate structures.

U2 - 10.1038/s41467-023-42669-6

DO - 10.1038/s41467-023-42669-6

M3 - Journal article

C2 - 37919266

AN - SCOPUS:85175847515

VL - 14

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 7000

ER -

ID: 372964535