Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis. / Simonsen, S.; Brøgger, P.; Kezic, Sanja; Thyssen, Jacob P.; Skov, Lone.

In: Dermatology, Vol. 237, No. 6, 2021, p. 940-945.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Simonsen, S, Brøgger, P, Kezic, S, Thyssen, JP & Skov, L 2021, 'Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis', Dermatology, vol. 237, no. 6, pp. 940-945. https://doi.org/10.1159/000514308

APA

Simonsen, S., Brøgger, P., Kezic, S., Thyssen, J. P., & Skov, L. (2021). Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis. Dermatology, 237(6), 940-945. https://doi.org/10.1159/000514308

Vancouver

Simonsen S, Brøgger P, Kezic S, Thyssen JP, Skov L. Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis. Dermatology. 2021;237(6):940-945. https://doi.org/10.1159/000514308

Author

Simonsen, S. ; Brøgger, P. ; Kezic, Sanja ; Thyssen, Jacob P. ; Skov, Lone. / Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis. In: Dermatology. 2021 ; Vol. 237, No. 6. pp. 940-945.

Bibtex

@article{aa2d757fc9954253b709f7048b77a8f5,
title = "Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis",
abstract = "Skin biomarkers for disease severity and treatment response in atopic dermatitis (AD) are needed. Biopsies cause scarring and tape stripping represents an alternative minimally invasive method for stratum corneum sampling. In this study, we examined the gene expression of cytokines in skin biopsies and cytokines in stratum corneum tape strips collected from adults with AD. We collected punch biopsies and tape strips from healthy controls (n = 6) and subjects with AD (n = 12) at baseline and after 2 weeks of topical treatment with mometasone furoate 0.1% cream. We found that IFN-γ, IL-13, and IL-10 mRNA (biopsies) and IL-1β protein expression levels (tape strips) were significantly increased in lesional AD skin compared to healthy control skin. Treatment with topical corticosteroid led to a significant decrease in mRNA levels for IL-13 and IL-4R but no significant differences in cytokine protein levels measured in tape strips. Finally, we found no significant correlations between cytokine levels in tape strips and mRNA levels in skin biopsies. ",
keywords = "Atopic dermatitis, Immunology, Skin inflammation",
author = "S. Simonsen and P. Br{\o}gger and Sanja Kezic and Thyssen, {Jacob P.} and Lone Skov",
note = "Publisher Copyright: {\textcopyright} 2021 S. Karger AG. All rights reserved.",
year = "2021",
doi = "10.1159/000514308",
language = "English",
volume = "237",
pages = "940--945",
journal = "Dermatology",
issn = "1018-8665",
publisher = "S Karger AG",
number = "6",

}

RIS

TY - JOUR

T1 - Comparison of Cytokines in Skin Biopsies and Tape Strips from Adults with Atopic Dermatitis

AU - Simonsen, S.

AU - Brøgger, P.

AU - Kezic, Sanja

AU - Thyssen, Jacob P.

AU - Skov, Lone

N1 - Publisher Copyright: © 2021 S. Karger AG. All rights reserved.

PY - 2021

Y1 - 2021

N2 - Skin biomarkers for disease severity and treatment response in atopic dermatitis (AD) are needed. Biopsies cause scarring and tape stripping represents an alternative minimally invasive method for stratum corneum sampling. In this study, we examined the gene expression of cytokines in skin biopsies and cytokines in stratum corneum tape strips collected from adults with AD. We collected punch biopsies and tape strips from healthy controls (n = 6) and subjects with AD (n = 12) at baseline and after 2 weeks of topical treatment with mometasone furoate 0.1% cream. We found that IFN-γ, IL-13, and IL-10 mRNA (biopsies) and IL-1β protein expression levels (tape strips) were significantly increased in lesional AD skin compared to healthy control skin. Treatment with topical corticosteroid led to a significant decrease in mRNA levels for IL-13 and IL-4R but no significant differences in cytokine protein levels measured in tape strips. Finally, we found no significant correlations between cytokine levels in tape strips and mRNA levels in skin biopsies.

AB - Skin biomarkers for disease severity and treatment response in atopic dermatitis (AD) are needed. Biopsies cause scarring and tape stripping represents an alternative minimally invasive method for stratum corneum sampling. In this study, we examined the gene expression of cytokines in skin biopsies and cytokines in stratum corneum tape strips collected from adults with AD. We collected punch biopsies and tape strips from healthy controls (n = 6) and subjects with AD (n = 12) at baseline and after 2 weeks of topical treatment with mometasone furoate 0.1% cream. We found that IFN-γ, IL-13, and IL-10 mRNA (biopsies) and IL-1β protein expression levels (tape strips) were significantly increased in lesional AD skin compared to healthy control skin. Treatment with topical corticosteroid led to a significant decrease in mRNA levels for IL-13 and IL-4R but no significant differences in cytokine protein levels measured in tape strips. Finally, we found no significant correlations between cytokine levels in tape strips and mRNA levels in skin biopsies.

KW - Atopic dermatitis

KW - Immunology

KW - Skin inflammation

U2 - 10.1159/000514308

DO - 10.1159/000514308

M3 - Journal article

C2 - 33971652

AN - SCOPUS:85107496157

VL - 237

SP - 940

EP - 945

JO - Dermatology

JF - Dermatology

SN - 1018-8665

IS - 6

ER -

ID: 304364167