Circulating basophils in patients with type IIb autoimmune chronic spontaneous urticaria have a lower histamine content
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Circulating basophils in patients with type IIb autoimmune chronic spontaneous urticaria have a lower histamine content. / Baumann, Katrine; Sørensen, Jennifer Astrup; Zhang, Ditte G; Ghazanfar, Misbah N; Skov, Per Stahl; Woetmann, Anders; Thomsen, Simon Francis.
In: JEADV Clinical Practice, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Circulating basophils in patients with type IIb autoimmune chronic spontaneous urticaria have a lower histamine content
AU - Baumann, Katrine
AU - Sørensen, Jennifer Astrup
AU - Zhang, Ditte G
AU - Ghazanfar, Misbah N
AU - Skov, Per Stahl
AU - Woetmann, Anders
AU - Thomsen, Simon Francis
N1 - Publisher Copyright: © 2024 The Authors. JEADV Clinical Practice published by John Wiley & Sons Ltd on behalf of European Academy of Dermatology and Venereology.
PY - 2024
Y1 - 2024
N2 - Background: Patients suffering from chronic spontaneous urticaria (CSU) are typically classified as type I or type IIb autoimmune CSU, but further patient stratification is hindered by the lack of biomarkers. Objectives: We investigated whether the histamine content of individual basophils differ between patient subtypes in CSU to evaluate its potential as a biomarker. Methods: A total of 101 patients diagnosed with CSU were included in the study. The histamine content per circulating basophil was derived from the basophil count in peripheral blood and levels of total cellular blood histamine. These measures, together with results from the serum-induced basophil histamine release assay (s-BHRA), were correlated to information on demographics, clinical characteristics, patient reported outcomes and laboratory analyses. Results: The histamine content per basophil was significantly different between s-BHRA negative and -positive patients (0.175 vs. 1.40 pg/cell, p < 0.001) and showed a significant negative correlation to the degree of basophil activation in s-BHRA (ρ = −0.209, p = 0.036). Furthermore, the amount of histamine in individual basophils was found to be significantly correlated with levels of total cellular blood histamine (ρ = 0.376, p < 0.001), eosinophil counts (ρ = 0.205, p = 0.040), levels of thyroid stimulating hormone (ρ = −0.246, p = 0.014) and titre of antibodies against thyroid peroxidase (ρ = −0.216, p = 0.031) and thyroglobulin (ρ = −0.203, p = 0.044). Conclusions: Low content of intracellular histamine in circulating basophils is associated with known markers of type IIb autoimmune CSU. Further studies are required to assess whether the amount of histamine in basophils can be used to monitor or predict response to treatment in patients.
AB - Background: Patients suffering from chronic spontaneous urticaria (CSU) are typically classified as type I or type IIb autoimmune CSU, but further patient stratification is hindered by the lack of biomarkers. Objectives: We investigated whether the histamine content of individual basophils differ between patient subtypes in CSU to evaluate its potential as a biomarker. Methods: A total of 101 patients diagnosed with CSU were included in the study. The histamine content per circulating basophil was derived from the basophil count in peripheral blood and levels of total cellular blood histamine. These measures, together with results from the serum-induced basophil histamine release assay (s-BHRA), were correlated to information on demographics, clinical characteristics, patient reported outcomes and laboratory analyses. Results: The histamine content per basophil was significantly different between s-BHRA negative and -positive patients (0.175 vs. 1.40 pg/cell, p < 0.001) and showed a significant negative correlation to the degree of basophil activation in s-BHRA (ρ = −0.209, p = 0.036). Furthermore, the amount of histamine in individual basophils was found to be significantly correlated with levels of total cellular blood histamine (ρ = 0.376, p < 0.001), eosinophil counts (ρ = 0.205, p = 0.040), levels of thyroid stimulating hormone (ρ = −0.246, p = 0.014) and titre of antibodies against thyroid peroxidase (ρ = −0.216, p = 0.031) and thyroglobulin (ρ = −0.203, p = 0.044). Conclusions: Low content of intracellular histamine in circulating basophils is associated with known markers of type IIb autoimmune CSU. Further studies are required to assess whether the amount of histamine in basophils can be used to monitor or predict response to treatment in patients.
KW - basophil histamine release assay
KW - basophils
KW - biomarkers
KW - chronic spontaneous urticaria
KW - histamine
KW - patient stratification
UR - http://www.scopus.com/inward/record.url?scp=85183050510&partnerID=8YFLogxK
U2 - 10.1002/jvc2.365
DO - 10.1002/jvc2.365
M3 - Journal article
AN - SCOPUS:85183050510
JO - JEADV Clinical Practice
JF - JEADV Clinical Practice
SN - 2768-6566
ER -
ID: 380749470