Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned piglets

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned piglets. / Fiil, Berthe Katrine; Thrane, Sandra Wingaard; Pichler, Michael; Kittilä, Tiia; Ledsgaard, Line; Ahmadi, Shirin; Hermansen, Grith Miriam Maigaard; Jelsbak, Lars; Lauridsen, Charlotte; Brix, Susanne; Laustsen, Andreas Hougaard.

In: iScience, Vol. 25, No. 4, 104003, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Fiil, BK, Thrane, SW, Pichler, M, Kittilä, T, Ledsgaard, L, Ahmadi, S, Hermansen, GMM, Jelsbak, L, Lauridsen, C, Brix, S & Laustsen, AH 2022, 'Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned piglets', iScience, vol. 25, no. 4, 104003. https://doi.org/10.1016/j.isci.2022.104003

APA

Fiil, B. K., Thrane, S. W., Pichler, M., Kittilä, T., Ledsgaard, L., Ahmadi, S., Hermansen, G. M. M., Jelsbak, L., Lauridsen, C., Brix, S., & Laustsen, A. H. (2022). Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned piglets. iScience, 25(4), [104003]. https://doi.org/10.1016/j.isci.2022.104003

Vancouver

Fiil BK, Thrane SW, Pichler M, Kittilä T, Ledsgaard L, Ahmadi S et al. Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned piglets. iScience. 2022;25(4). 104003. https://doi.org/10.1016/j.isci.2022.104003

Author

Fiil, Berthe Katrine ; Thrane, Sandra Wingaard ; Pichler, Michael ; Kittilä, Tiia ; Ledsgaard, Line ; Ahmadi, Shirin ; Hermansen, Grith Miriam Maigaard ; Jelsbak, Lars ; Lauridsen, Charlotte ; Brix, Susanne ; Laustsen, Andreas Hougaard. / Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned piglets. In: iScience. 2022 ; Vol. 25, No. 4.

Bibtex

@article{0c710e4201da4d659092541de0178d20,
title = "Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned piglets",
abstract = "A major challenge in industrial pig production is the prevalence of post-weaning diarrhea (PWD) in piglets, often caused by enterotoxigenic Escherichia coli (ETEC). The increased use of antibiotics and zinc oxide to treat PWD has raised global concerns regarding antimicrobial resistance development and environmental pollution. Still, alternative treatments targeting ETEC and counteracting PWD are largely lacking. Here, we report the design of a pH, temperature, and protease-stable bivalent VHH-based protein BL1.2 that cross-links a F4+ ETEC model strain by selectively binding to its fimbriae. This protein inhibits F4+ ETEC adhesion to porcine epithelial cells ex vivo and decreases F4+ ETEC proliferation when administrated as a feed additive to weaned F4+ ETEC challenged piglets. These findings highlight the potential of a highly specific bivalent VHH-based feed additive in effectively delimiting pathogenic F4+ ETEC bacteria proliferation in piglets and may represent a sustainable solution for managing PWD while circumventing antimicrobial resistance development.",
keywords = "Infection control in health technology, Microbiology, Porcine medicine",
author = "Fiil, {Berthe Katrine} and Thrane, {Sandra Wingaard} and Michael Pichler and Tiia Kittil{\"a} and Line Ledsgaard and Shirin Ahmadi and Hermansen, {Grith Miriam Maigaard} and Lars Jelsbak and Charlotte Lauridsen and Susanne Brix and Laustsen, {Andreas Hougaard}",
note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
doi = "10.1016/j.isci.2022.104003",
language = "English",
volume = "25",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - Orally active bivalent VHH construct prevents proliferation of F4+ enterotoxigenic Escherichia coli in weaned piglets

AU - Fiil, Berthe Katrine

AU - Thrane, Sandra Wingaard

AU - Pichler, Michael

AU - Kittilä, Tiia

AU - Ledsgaard, Line

AU - Ahmadi, Shirin

AU - Hermansen, Grith Miriam Maigaard

AU - Jelsbak, Lars

AU - Lauridsen, Charlotte

AU - Brix, Susanne

AU - Laustsen, Andreas Hougaard

N1 - Publisher Copyright: © 2022 The Author(s)

PY - 2022

Y1 - 2022

N2 - A major challenge in industrial pig production is the prevalence of post-weaning diarrhea (PWD) in piglets, often caused by enterotoxigenic Escherichia coli (ETEC). The increased use of antibiotics and zinc oxide to treat PWD has raised global concerns regarding antimicrobial resistance development and environmental pollution. Still, alternative treatments targeting ETEC and counteracting PWD are largely lacking. Here, we report the design of a pH, temperature, and protease-stable bivalent VHH-based protein BL1.2 that cross-links a F4+ ETEC model strain by selectively binding to its fimbriae. This protein inhibits F4+ ETEC adhesion to porcine epithelial cells ex vivo and decreases F4+ ETEC proliferation when administrated as a feed additive to weaned F4+ ETEC challenged piglets. These findings highlight the potential of a highly specific bivalent VHH-based feed additive in effectively delimiting pathogenic F4+ ETEC bacteria proliferation in piglets and may represent a sustainable solution for managing PWD while circumventing antimicrobial resistance development.

AB - A major challenge in industrial pig production is the prevalence of post-weaning diarrhea (PWD) in piglets, often caused by enterotoxigenic Escherichia coli (ETEC). The increased use of antibiotics and zinc oxide to treat PWD has raised global concerns regarding antimicrobial resistance development and environmental pollution. Still, alternative treatments targeting ETEC and counteracting PWD are largely lacking. Here, we report the design of a pH, temperature, and protease-stable bivalent VHH-based protein BL1.2 that cross-links a F4+ ETEC model strain by selectively binding to its fimbriae. This protein inhibits F4+ ETEC adhesion to porcine epithelial cells ex vivo and decreases F4+ ETEC proliferation when administrated as a feed additive to weaned F4+ ETEC challenged piglets. These findings highlight the potential of a highly specific bivalent VHH-based feed additive in effectively delimiting pathogenic F4+ ETEC bacteria proliferation in piglets and may represent a sustainable solution for managing PWD while circumventing antimicrobial resistance development.

KW - Infection control in health technology

KW - Microbiology

KW - Porcine medicine

U2 - 10.1016/j.isci.2022.104003

DO - 10.1016/j.isci.2022.104003

M3 - Journal article

C2 - 35310945

AN - SCOPUS:85127578491

VL - 25

JO - iScience

JF - iScience

SN - 2589-0042

IS - 4

M1 - 104003

ER -

ID: 339896177