The Thioredoxin-Interacting Protein TXNIP Is a Putative Tumour Suppressor in Cutaneous T-Cell Lymphoma

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Standard

The Thioredoxin-Interacting Protein TXNIP Is a Putative Tumour Suppressor in Cutaneous T-Cell Lymphoma. / Stolearenco, Veronica; Levring, Trine B; Nielsen, Helene Myrtue; Lindahl, Lise; Fredholm, Simon; Kongsbak-Wismann, Martin; Willerslev-Olsen, Andreas; Buus, Terkild B; Nastasi, Claudia; Hu, Tengpeng; Gluud, Maria; Côme, Christophe R M; Krejsgaard, Thorbjørn; Iversen, Lars; Bonefeld, Charlotte Menné; Grønbæk, Kirsten; Met, Özcan; Woetmann, Anders; Ødum, Niels; Geisler, Carsten.

In: Dermatology, Vol. 237, No. 2, 2021, p. 283-290.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Stolearenco, V, Levring, TB, Nielsen, HM, Lindahl, L, Fredholm, S, Kongsbak-Wismann, M, Willerslev-Olsen, A, Buus, TB, Nastasi, C, Hu, T, Gluud, M, Côme, CRM, Krejsgaard, T, Iversen, L, Bonefeld, CM, Grønbæk, K, Met, Ö, Woetmann, A, Ødum, N & Geisler, C 2021, 'The Thioredoxin-Interacting Protein TXNIP Is a Putative Tumour Suppressor in Cutaneous T-Cell Lymphoma', Dermatology, vol. 237, no. 2, pp. 283-290. https://doi.org/10.1159/000509159

APA

Stolearenco, V., Levring, T. B., Nielsen, H. M., Lindahl, L., Fredholm, S., Kongsbak-Wismann, M., Willerslev-Olsen, A., Buus, T. B., Nastasi, C., Hu, T., Gluud, M., Côme, C. R. M., Krejsgaard, T., Iversen, L., Bonefeld, C. M., Grønbæk, K., Met, Ö., Woetmann, A., Ødum, N., & Geisler, C. (2021). The Thioredoxin-Interacting Protein TXNIP Is a Putative Tumour Suppressor in Cutaneous T-Cell Lymphoma. Dermatology, 237(2), 283-290. https://doi.org/10.1159/000509159

Vancouver

Stolearenco V, Levring TB, Nielsen HM, Lindahl L, Fredholm S, Kongsbak-Wismann M et al. The Thioredoxin-Interacting Protein TXNIP Is a Putative Tumour Suppressor in Cutaneous T-Cell Lymphoma. Dermatology. 2021;237(2):283-290. https://doi.org/10.1159/000509159

Author

Stolearenco, Veronica ; Levring, Trine B ; Nielsen, Helene Myrtue ; Lindahl, Lise ; Fredholm, Simon ; Kongsbak-Wismann, Martin ; Willerslev-Olsen, Andreas ; Buus, Terkild B ; Nastasi, Claudia ; Hu, Tengpeng ; Gluud, Maria ; Côme, Christophe R M ; Krejsgaard, Thorbjørn ; Iversen, Lars ; Bonefeld, Charlotte Menné ; Grønbæk, Kirsten ; Met, Özcan ; Woetmann, Anders ; Ødum, Niels ; Geisler, Carsten. / The Thioredoxin-Interacting Protein TXNIP Is a Putative Tumour Suppressor in Cutaneous T-Cell Lymphoma. In: Dermatology. 2021 ; Vol. 237, No. 2. pp. 283-290.

Bibtex

@article{ea5e1d02690f446ab5bcb13543c4b7f7,
title = "The Thioredoxin-Interacting Protein TXNIP Is a Putative Tumour Suppressor in Cutaneous T-Cell Lymphoma",
abstract = "BACKGROUND: The thioredoxin-interacting protein (TXNIP) is involved in cellular metabolism and cell proliferation, and recently, deficient expression of TXNIP has been associated with progression and poor outcome for cancer patients.OBJECTIVES: To assess TXNIP expression and function in malignant T cells from cutaneous T-cell lymphoma (CTCL).METHODS: CTCL-derived malignant (MyLa2059, PB2B) and non-malignant (MyLa1850) cell lines were analysed by Western blotting and qPCR for TXNIP expression. Subsequently, the malignant CTCL cell lines were treated with GSK126 - an inhibitor of enhancer of zeste homolog 2 (EZH2) methyltransferase activity or assessed by bisulphite sequencing for TXNIP promoter methylation. Methylation was also assessed with the demethylating agent 5-azacytidine (5AZA). Finally, TXNIP was overexpressed in the malignant PB2B cell line via plasmid transduction, and the effect of TXNIP was further analysed by flow cytometry.RESULTS: We report on low expression of TXNIP protein in all cell lines representing different subtypes and stages of CTCL when compared to non-malignant T cells. Epigenetic silencing and other mechanisms were involved in the repression of TXNIP whereas forced expression of TXNIP strongly inhibited proliferation of malignant T cells.CONCLUSIONS: Epigenetic silencing and other as yet unknown mechanisms repress TXNIP expression in malignant T cells. As forced expression of TXNIP inhibits malignant proliferation, we propose that TXNIP is a putative tumour suppressor in CTCL.",
author = "Veronica Stolearenco and Levring, {Trine B} and Nielsen, {Helene Myrtue} and Lise Lindahl and Simon Fredholm and Martin Kongsbak-Wismann and Andreas Willerslev-Olsen and Buus, {Terkild B} and Claudia Nastasi and Tengpeng Hu and Maria Gluud and C{\^o}me, {Christophe R M} and Thorbj{\o}rn Krejsgaard and Lars Iversen and Bonefeld, {Charlotte Menn{\'e}} and Kirsten Gr{\o}nb{\ae}k and {\"O}zcan Met and Anders Woetmann and Niels {\O}dum and Carsten Geisler",
note = "{\textcopyright} 2020 S. Karger AG, Basel.",
year = "2021",
doi = "10.1159/000509159",
language = "English",
volume = "237",
pages = "283--290",
journal = "Dermatology",
issn = "1018-8665",
publisher = "S Karger AG",
number = "2",

}

RIS

TY - JOUR

T1 - The Thioredoxin-Interacting Protein TXNIP Is a Putative Tumour Suppressor in Cutaneous T-Cell Lymphoma

AU - Stolearenco, Veronica

AU - Levring, Trine B

AU - Nielsen, Helene Myrtue

AU - Lindahl, Lise

AU - Fredholm, Simon

AU - Kongsbak-Wismann, Martin

AU - Willerslev-Olsen, Andreas

AU - Buus, Terkild B

AU - Nastasi, Claudia

AU - Hu, Tengpeng

AU - Gluud, Maria

AU - Côme, Christophe R M

AU - Krejsgaard, Thorbjørn

AU - Iversen, Lars

AU - Bonefeld, Charlotte Menné

AU - Grønbæk, Kirsten

AU - Met, Özcan

AU - Woetmann, Anders

AU - Ødum, Niels

AU - Geisler, Carsten

N1 - © 2020 S. Karger AG, Basel.

PY - 2021

Y1 - 2021

N2 - BACKGROUND: The thioredoxin-interacting protein (TXNIP) is involved in cellular metabolism and cell proliferation, and recently, deficient expression of TXNIP has been associated with progression and poor outcome for cancer patients.OBJECTIVES: To assess TXNIP expression and function in malignant T cells from cutaneous T-cell lymphoma (CTCL).METHODS: CTCL-derived malignant (MyLa2059, PB2B) and non-malignant (MyLa1850) cell lines were analysed by Western blotting and qPCR for TXNIP expression. Subsequently, the malignant CTCL cell lines were treated with GSK126 - an inhibitor of enhancer of zeste homolog 2 (EZH2) methyltransferase activity or assessed by bisulphite sequencing for TXNIP promoter methylation. Methylation was also assessed with the demethylating agent 5-azacytidine (5AZA). Finally, TXNIP was overexpressed in the malignant PB2B cell line via plasmid transduction, and the effect of TXNIP was further analysed by flow cytometry.RESULTS: We report on low expression of TXNIP protein in all cell lines representing different subtypes and stages of CTCL when compared to non-malignant T cells. Epigenetic silencing and other mechanisms were involved in the repression of TXNIP whereas forced expression of TXNIP strongly inhibited proliferation of malignant T cells.CONCLUSIONS: Epigenetic silencing and other as yet unknown mechanisms repress TXNIP expression in malignant T cells. As forced expression of TXNIP inhibits malignant proliferation, we propose that TXNIP is a putative tumour suppressor in CTCL.

AB - BACKGROUND: The thioredoxin-interacting protein (TXNIP) is involved in cellular metabolism and cell proliferation, and recently, deficient expression of TXNIP has been associated with progression and poor outcome for cancer patients.OBJECTIVES: To assess TXNIP expression and function in malignant T cells from cutaneous T-cell lymphoma (CTCL).METHODS: CTCL-derived malignant (MyLa2059, PB2B) and non-malignant (MyLa1850) cell lines were analysed by Western blotting and qPCR for TXNIP expression. Subsequently, the malignant CTCL cell lines were treated with GSK126 - an inhibitor of enhancer of zeste homolog 2 (EZH2) methyltransferase activity or assessed by bisulphite sequencing for TXNIP promoter methylation. Methylation was also assessed with the demethylating agent 5-azacytidine (5AZA). Finally, TXNIP was overexpressed in the malignant PB2B cell line via plasmid transduction, and the effect of TXNIP was further analysed by flow cytometry.RESULTS: We report on low expression of TXNIP protein in all cell lines representing different subtypes and stages of CTCL when compared to non-malignant T cells. Epigenetic silencing and other mechanisms were involved in the repression of TXNIP whereas forced expression of TXNIP strongly inhibited proliferation of malignant T cells.CONCLUSIONS: Epigenetic silencing and other as yet unknown mechanisms repress TXNIP expression in malignant T cells. As forced expression of TXNIP inhibits malignant proliferation, we propose that TXNIP is a putative tumour suppressor in CTCL.

U2 - 10.1159/000509159

DO - 10.1159/000509159

M3 - Journal article

C2 - 32799209

VL - 237

SP - 283

EP - 290

JO - Dermatology

JF - Dermatology

SN - 1018-8665

IS - 2

ER -

ID: 247510965