The immune privilege of the eye: human retinal pigment epithelial cells selectively modulate T-cell activation in vitro.
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The immune privilege of the eye: human retinal pigment epithelial cells selectively modulate T-cell activation in vitro. / Kaestel, Charlotte G; Lovato, Paola; Ødum, Niels; Nissen, Mogens H; Röpke, Carsten.
In: Current Eye Research, Vol. 30, No. 5, 2005, p. 375-83.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The immune privilege of the eye: human retinal pigment epithelial cells selectively modulate T-cell activation in vitro.
AU - Kaestel, Charlotte G
AU - Lovato, Paola
AU - Ødum, Niels
AU - Nissen, Mogens H
AU - Röpke, Carsten
N1 - Keywords: Antigens, CD; Antigens, CD3; Antigens, Differentiation, T-Lymphocyte; Cell Cycle; Cell Line; Cell Proliferation; Coculture Techniques; DNA Replication; Enzyme-Linked Immunosorbent Assay; Eye; Flow Cytometry; Histocompatibility Antigens Class I; Histocompatibility Antigens Class II; Humans; Immune System; Interleukin-2 Receptor alpha Subunit; Lymphocyte Activation; Pigment Epithelium of Eye; Receptors, Interleukin; T-Lymphocytes
PY - 2005
Y1 - 2005
N2 - PURPOSE: To examine the effect of human retinal pigment epithelial (RPE) cells on phytohemagglutinin (PHA) activation of T cells. METHODS: Resting peripheral blood lymphocytes (PBLs) were stimulated with PHA with or without the presence of gamma-irradiated RPE cells. Proliferation and the cell cycle profile were thereafter investigated by 3H-thymidine incorporation and flow cytometric analysis. In addition, the PBLs expression of CD69, major histocompatibility complex (MHC) class I and II, CD3, as well as the IL-2 receptor chains were evaluated by flow cytometry, and the content of IL-2 in cell culture supernatant was measured by ELISA. RESULTS: Human RPE cells were found to suppress PHA-induced proliferation, cyclin A, IL-2R-alpha and -gamma, and CD71 expression and decrease the production of IL-2; but RPE cells do not inhibit the PHA-induced expression of early activation markers CD69, MHC class I and II, and of cyclin D of the PBLs. CONCLUSIONS: These results are the first to indicate that RPE cells impede generation of activated T cells by interfering with the induction of high-affinity IL-2R-alphabetagamma, IL-2 production, and the expression of CD71 and cyclin A.
AB - PURPOSE: To examine the effect of human retinal pigment epithelial (RPE) cells on phytohemagglutinin (PHA) activation of T cells. METHODS: Resting peripheral blood lymphocytes (PBLs) were stimulated with PHA with or without the presence of gamma-irradiated RPE cells. Proliferation and the cell cycle profile were thereafter investigated by 3H-thymidine incorporation and flow cytometric analysis. In addition, the PBLs expression of CD69, major histocompatibility complex (MHC) class I and II, CD3, as well as the IL-2 receptor chains were evaluated by flow cytometry, and the content of IL-2 in cell culture supernatant was measured by ELISA. RESULTS: Human RPE cells were found to suppress PHA-induced proliferation, cyclin A, IL-2R-alpha and -gamma, and CD71 expression and decrease the production of IL-2; but RPE cells do not inhibit the PHA-induced expression of early activation markers CD69, MHC class I and II, and of cyclin D of the PBLs. CONCLUSIONS: These results are the first to indicate that RPE cells impede generation of activated T cells by interfering with the induction of high-affinity IL-2R-alphabetagamma, IL-2 production, and the expression of CD71 and cyclin A.
U2 - 10.1080/02713680590934120
DO - 10.1080/02713680590934120
M3 - Journal article
C2 - 16020268
VL - 30
SP - 375
EP - 383
JO - Current Eye Research
JF - Current Eye Research
SN - 0271-3683
IS - 5
ER -
ID: 8724898