The Escherichia coli protein toxin cytotoxic necrotizing factor 1 induces epithelial mesenchymal transition
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The Escherichia coli protein toxin cytotoxic necrotizing factor 1 induces epithelial mesenchymal transition. / Fabbri, Alessia; Travaglione, Sara; Rosadi, Francesca; Ballan, Giulia; Maroccia, Zaira; Giambenedetti, Massimo; Guidotti, Marco; Ødum, Niels; Krejsgaard, Thorbjørn; Fiorentini, Carla.
In: Cellular Microbiology, Vol. 22, No. 2, e13138, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The Escherichia coli protein toxin cytotoxic necrotizing factor 1 induces epithelial mesenchymal transition
AU - Fabbri, Alessia
AU - Travaglione, Sara
AU - Rosadi, Francesca
AU - Ballan, Giulia
AU - Maroccia, Zaira
AU - Giambenedetti, Massimo
AU - Guidotti, Marco
AU - Ødum, Niels
AU - Krejsgaard, Thorbjørn
AU - Fiorentini, Carla
PY - 2020
Y1 - 2020
N2 - Some toxigenic bacteria produce protein toxins with carcinogenic signatures, which either directly damage DNA or stimulate signalling pathways related to cancer. So far, however, only a few of them have been proved to favour the induction or progression of cancer. In this work, we report that the Rho-activating Escherichia coli protein toxin, cytotoxic necrotising factor 1 (CNF1), induces epithelial to mesenchymal transition (EMT) in intestinal epithelial cells. EMT is a crucial step in malignant tumour conversion and invasiveness. In the case of CNF1, it occurs by up-regulation of the transcription factors ZEB1 and Snail1, delocalisation of E-cadherin and β-catenin, activation of the serine/threonine kinase mTOR, accelerated wound healing, and invasion. However, our results highlight that nontransformed epithelial cells entail the presence of inflammatory factors, in addition to CNF1, to acquire a mesenchymal-like behaviour. All this suggests that the surrounding microenvironment, as well as the cell type, dramatically influences the CNF1 ability to promote carcinogenic traits.
AB - Some toxigenic bacteria produce protein toxins with carcinogenic signatures, which either directly damage DNA or stimulate signalling pathways related to cancer. So far, however, only a few of them have been proved to favour the induction or progression of cancer. In this work, we report that the Rho-activating Escherichia coli protein toxin, cytotoxic necrotising factor 1 (CNF1), induces epithelial to mesenchymal transition (EMT) in intestinal epithelial cells. EMT is a crucial step in malignant tumour conversion and invasiveness. In the case of CNF1, it occurs by up-regulation of the transcription factors ZEB1 and Snail1, delocalisation of E-cadherin and β-catenin, activation of the serine/threonine kinase mTOR, accelerated wound healing, and invasion. However, our results highlight that nontransformed epithelial cells entail the presence of inflammatory factors, in addition to CNF1, to acquire a mesenchymal-like behaviour. All this suggests that the surrounding microenvironment, as well as the cell type, dramatically influences the CNF1 ability to promote carcinogenic traits.
KW - cancer
KW - mTOR
KW - nontransformed cell
KW - Rho GTPases
KW - transformed cell
KW - virulence factors
U2 - 10.1111/cmi.13138
DO - 10.1111/cmi.13138
M3 - Journal article
C2 - 31698538
AN - SCOPUS:85075477741
VL - 22
JO - Cellular Microbiology
JF - Cellular Microbiology
SN - 1462-5814
IS - 2
M1 - e13138
ER -
ID: 235589642