Substoichiometric ribose methylations in spliceosomal snRNAs

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Substoichiometric ribose methylations in spliceosomal snRNAs. / Krogh, Nicolai; Kongsbak-Wismann, Martin; Geisler, Carsten; Nielsen, Henrik.

In: Organic and Biomolecular Chemistry, Vol. 15, No. 42, 2017, p. 8872-8876.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Krogh, N, Kongsbak-Wismann, M, Geisler, C & Nielsen, H 2017, 'Substoichiometric ribose methylations in spliceosomal snRNAs', Organic and Biomolecular Chemistry, vol. 15, no. 42, pp. 8872-8876. https://doi.org/10.1039/c7ob02317k

APA

Krogh, N., Kongsbak-Wismann, M., Geisler, C., & Nielsen, H. (2017). Substoichiometric ribose methylations in spliceosomal snRNAs. Organic and Biomolecular Chemistry, 15(42), 8872-8876. https://doi.org/10.1039/c7ob02317k

Vancouver

Krogh N, Kongsbak-Wismann M, Geisler C, Nielsen H. Substoichiometric ribose methylations in spliceosomal snRNAs. Organic and Biomolecular Chemistry. 2017;15(42):8872-8876. https://doi.org/10.1039/c7ob02317k

Author

Krogh, Nicolai ; Kongsbak-Wismann, Martin ; Geisler, Carsten ; Nielsen, Henrik. / Substoichiometric ribose methylations in spliceosomal snRNAs. In: Organic and Biomolecular Chemistry. 2017 ; Vol. 15, No. 42. pp. 8872-8876.

Bibtex

@article{23fa46e852d94ff38d1145034af56a20,
title = "Substoichiometric ribose methylations in spliceosomal snRNAs",
abstract = "Sequencing-based profiling of ribose methylations is a new approach that allows for experiments addressing dynamic changes on a large scale. Here, we apply such a method to spliceosomal snRNAs present in human whole cell RNA. Analysis of solid tissue samples confirmed all previously known sites and demonstrated close to full methylation at almost all sites. Methylation changes were revealed in biological experimental settings, using T cell activation as an example, and in the T cell leukemia model, Jurkat cells. Such changes could impact the dynamics of snRNA interactions during the spliceosome cycle and affect mRNA splicing efficiency and splicing patterns.",
author = "Nicolai Krogh and Martin Kongsbak-Wismann and Carsten Geisler and Henrik Nielsen",
year = "2017",
doi = "10.1039/c7ob02317k",
language = "English",
volume = "15",
pages = "8872--8876",
journal = "Organic & Biomolecular Chemistry",
issn = "1470-4358",
publisher = "Royal Society of Chemistry",
number = "42",

}

RIS

TY - JOUR

T1 - Substoichiometric ribose methylations in spliceosomal snRNAs

AU - Krogh, Nicolai

AU - Kongsbak-Wismann, Martin

AU - Geisler, Carsten

AU - Nielsen, Henrik

PY - 2017

Y1 - 2017

N2 - Sequencing-based profiling of ribose methylations is a new approach that allows for experiments addressing dynamic changes on a large scale. Here, we apply such a method to spliceosomal snRNAs present in human whole cell RNA. Analysis of solid tissue samples confirmed all previously known sites and demonstrated close to full methylation at almost all sites. Methylation changes were revealed in biological experimental settings, using T cell activation as an example, and in the T cell leukemia model, Jurkat cells. Such changes could impact the dynamics of snRNA interactions during the spliceosome cycle and affect mRNA splicing efficiency and splicing patterns.

AB - Sequencing-based profiling of ribose methylations is a new approach that allows for experiments addressing dynamic changes on a large scale. Here, we apply such a method to spliceosomal snRNAs present in human whole cell RNA. Analysis of solid tissue samples confirmed all previously known sites and demonstrated close to full methylation at almost all sites. Methylation changes were revealed in biological experimental settings, using T cell activation as an example, and in the T cell leukemia model, Jurkat cells. Such changes could impact the dynamics of snRNA interactions during the spliceosome cycle and affect mRNA splicing efficiency and splicing patterns.

U2 - 10.1039/c7ob02317k

DO - 10.1039/c7ob02317k

M3 - Journal article

C2 - 29048444

AN - SCOPUS:85032730824

VL - 15

SP - 8872

EP - 8876

JO - Organic & Biomolecular Chemistry

JF - Organic & Biomolecular Chemistry

SN - 1470-4358

IS - 42

ER -

ID: 185720536