STAT3 activation and infiltration of eosinophil granulocytes in mycosis fungoides
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STAT3 activation and infiltration of eosinophil granulocytes in mycosis fungoides. / Fredholm, Simon; Gjerdrum, Lise Mette R; Willerslev-Olsen, Andreas; Petersen, David L; Nielsen, Inger Ø; Kauczok, Claudia-S; Wobser, Marion; Ralfkiaer, Ulrik; Bonefeld, Charlotte M; Wasik, Mariusz A; Krejsgaard, Thorbjørn; Geisler, Carsten; Ralfkiaer, Elisabeth; Gniadecki, Robert; Andersen, Anders Woetmann; Odum, Niels.
In: Anticancer Research, Vol. 34, No. 10, 2014, p. 5277-5286.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - STAT3 activation and infiltration of eosinophil granulocytes in mycosis fungoides
AU - Fredholm, Simon
AU - Gjerdrum, Lise Mette R
AU - Willerslev-Olsen, Andreas
AU - Petersen, David L
AU - Nielsen, Inger Ø
AU - Kauczok, Claudia-S
AU - Wobser, Marion
AU - Ralfkiaer, Ulrik
AU - Bonefeld, Charlotte M
AU - Wasik, Mariusz A
AU - Krejsgaard, Thorbjørn
AU - Geisler, Carsten
AU - Ralfkiaer, Elisabeth
AU - Gniadecki, Robert
AU - Andersen, Anders Woetmann
AU - Odum, Niels
N1 - Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
PY - 2014
Y1 - 2014
N2 - Eosinophil granulocytes have been implicated in anticancer immunity but recent data indicate that eosinophils can also promote cancer. Herein, we studied eosinophils in skin lesions from 43 patients with mycosis fungoides (MF). The presence of eosinophils correlated with disease stage: 78% of patients with advanced disease displayed eosinophil infiltration, whereas this was only seen in 11% of patients with patches (p<0.01), and in 48% of those with plaque disease. Importantly, 72% of patients with positive staining for phospho-signal-transducer-and-activator-of-transcription (pY-STAT3) in malignant T-cells also stained positively for eosinophils, whereas this was only observed in 28% of pY-STAT3-negative patients (p<0.01). Notably, malignant T-cells expressed eosinophilic activation and trafficking factors: High-mobility group BOX-1 protein (HMGB1) and interleukin 5 (IL5). STAT3 siRNA profoundly inhibited IL5 but not HMGB1 expression. In conclusion, these data suggest that malignant T-cells orchestrate accumulation and activation of eosinophils supporting the notion of STAT3 being a putative target for therapy.
AB - Eosinophil granulocytes have been implicated in anticancer immunity but recent data indicate that eosinophils can also promote cancer. Herein, we studied eosinophils in skin lesions from 43 patients with mycosis fungoides (MF). The presence of eosinophils correlated with disease stage: 78% of patients with advanced disease displayed eosinophil infiltration, whereas this was only seen in 11% of patients with patches (p<0.01), and in 48% of those with plaque disease. Importantly, 72% of patients with positive staining for phospho-signal-transducer-and-activator-of-transcription (pY-STAT3) in malignant T-cells also stained positively for eosinophils, whereas this was only observed in 28% of pY-STAT3-negative patients (p<0.01). Notably, malignant T-cells expressed eosinophilic activation and trafficking factors: High-mobility group BOX-1 protein (HMGB1) and interleukin 5 (IL5). STAT3 siRNA profoundly inhibited IL5 but not HMGB1 expression. In conclusion, these data suggest that malignant T-cells orchestrate accumulation and activation of eosinophils supporting the notion of STAT3 being a putative target for therapy.
KW - Biopsy
KW - Cell Line, Tumor
KW - Eosinophils
KW - Gene Knockout Techniques
KW - HMGB1 Protein
KW - Humans
KW - Immunohistochemistry
KW - Interleukin-5
KW - Mycosis Fungoides
KW - RNA Interference
KW - STAT3 Transcription Factor
KW - T-Lymphocytes
M3 - Journal article
C2 - 25275020
VL - 34
SP - 5277
EP - 5286
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 10
ER -
ID: 135228213