Specific depletion of mature T lymphocytes from human bone marrow

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Specific depletion of mature T lymphocytes from human bone marrow. / Geisler, C; Møller, J; Plesner, T; Dickmeiss, E; Pallesen, G; Larsen, J K; Jacobsen, N; Svejgaard, A.

In: Scandinavian Journal of Immunology, Vol. 29, No. 5, 1989, p. 617-25.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Geisler, C, Møller, J, Plesner, T, Dickmeiss, E, Pallesen, G, Larsen, JK, Jacobsen, N & Svejgaard, A 1989, 'Specific depletion of mature T lymphocytes from human bone marrow', Scandinavian Journal of Immunology, vol. 29, no. 5, pp. 617-25.

APA

Geisler, C., Møller, J., Plesner, T., Dickmeiss, E., Pallesen, G., Larsen, J. K., Jacobsen, N., & Svejgaard, A. (1989). Specific depletion of mature T lymphocytes from human bone marrow. Scandinavian Journal of Immunology, 29(5), 617-25.

Vancouver

Geisler C, Møller J, Plesner T, Dickmeiss E, Pallesen G, Larsen JK et al. Specific depletion of mature T lymphocytes from human bone marrow. Scandinavian Journal of Immunology. 1989;29(5):617-25.

Author

Geisler, C ; Møller, J ; Plesner, T ; Dickmeiss, E ; Pallesen, G ; Larsen, J K ; Jacobsen, N ; Svejgaard, A. / Specific depletion of mature T lymphocytes from human bone marrow. In: Scandinavian Journal of Immunology. 1989 ; Vol. 29, No. 5. pp. 617-25.

Bibtex

@article{fc36ff80b0a811ddb538000ea68e967b,
title = "Specific depletion of mature T lymphocytes from human bone marrow",
abstract = "An effective method for specific depletion of mature T lymphocytes from human bone marrow mononuclear cells (BMMC) with preservation of prethymic T cells and natural killer (NK) cells is presented. The BMMC were incubated with F101.01, a monoclonal antibody recognizing an epitope of the T-cell receptor-CD3 complex, and subsequently with immunomagnetic beads. Flow cytometric analysis demonstrated that mature T cells were efficiently depleted and that NK cells and prethymic T cells were preserved in the BMMC. Furthermore, T cell-mediated immune reaction as measured by thymidine incorporation after stimulation with phytohaemagglutinin was abolished, whereas NK activity as measured by 51Cr release using K562 as target cells was preserved. Recovery of colony-forming units of granulocyte-macrophages was 60-70%.",
author = "C Geisler and J M{\o}ller and T Plesner and E Dickmeiss and G Pallesen and Larsen, {J K} and N Jacobsen and A Svejgaard",
note = "Keywords: Bone Marrow; Bone Marrow Cells; Cell Separation; Colony-Forming Units Assay; Flow Cytometry; Hematopoietic Stem Cells; Humans; Killer Cells, Natural; Leukocyte Count; Lymphocyte Activation; Lymphocyte Depletion; T-Lymphocytes",
year = "1989",
language = "English",
volume = "29",
pages = "617--25",
journal = "Scandinavian Journal of Immunology, Supplement",
issn = "0301-6323",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - Specific depletion of mature T lymphocytes from human bone marrow

AU - Geisler, C

AU - Møller, J

AU - Plesner, T

AU - Dickmeiss, E

AU - Pallesen, G

AU - Larsen, J K

AU - Jacobsen, N

AU - Svejgaard, A

N1 - Keywords: Bone Marrow; Bone Marrow Cells; Cell Separation; Colony-Forming Units Assay; Flow Cytometry; Hematopoietic Stem Cells; Humans; Killer Cells, Natural; Leukocyte Count; Lymphocyte Activation; Lymphocyte Depletion; T-Lymphocytes

PY - 1989

Y1 - 1989

N2 - An effective method for specific depletion of mature T lymphocytes from human bone marrow mononuclear cells (BMMC) with preservation of prethymic T cells and natural killer (NK) cells is presented. The BMMC were incubated with F101.01, a monoclonal antibody recognizing an epitope of the T-cell receptor-CD3 complex, and subsequently with immunomagnetic beads. Flow cytometric analysis demonstrated that mature T cells were efficiently depleted and that NK cells and prethymic T cells were preserved in the BMMC. Furthermore, T cell-mediated immune reaction as measured by thymidine incorporation after stimulation with phytohaemagglutinin was abolished, whereas NK activity as measured by 51Cr release using K562 as target cells was preserved. Recovery of colony-forming units of granulocyte-macrophages was 60-70%.

AB - An effective method for specific depletion of mature T lymphocytes from human bone marrow mononuclear cells (BMMC) with preservation of prethymic T cells and natural killer (NK) cells is presented. The BMMC were incubated with F101.01, a monoclonal antibody recognizing an epitope of the T-cell receptor-CD3 complex, and subsequently with immunomagnetic beads. Flow cytometric analysis demonstrated that mature T cells were efficiently depleted and that NK cells and prethymic T cells were preserved in the BMMC. Furthermore, T cell-mediated immune reaction as measured by thymidine incorporation after stimulation with phytohaemagglutinin was abolished, whereas NK activity as measured by 51Cr release using K562 as target cells was preserved. Recovery of colony-forming units of granulocyte-macrophages was 60-70%.

M3 - Journal article

C2 - 2786245

VL - 29

SP - 617

EP - 625

JO - Scandinavian Journal of Immunology, Supplement

JF - Scandinavian Journal of Immunology, Supplement

SN - 0301-6323

IS - 5

ER -

ID: 8546549