TY - JOUR

T1 - Skin microdialysis

T2 - Methods, applications and future opportunities - An EAACI position paper

AU - Baumann, Katrine Y.

AU - Church, Martin K.

AU - Clough, Geraldine F.

AU - Quist, Sven Roy

AU - Schmelz, Martin

AU - Skov, Per Stahl

AU - Anderson, Chris D.

AU - Tannert, Line Kring

AU - Giménez-Arnau, Ana Maria

AU - Frischbutter, Stefan

AU - Scheffel, Jörg

AU - Maurer, Marcus

PY - 2019

Y1 - 2019

N2 - Skin microdialysis (SMD) is a versatile sampling technique that can be used to recover soluble endogenous and exogenous molecules from the extracellular compartment of human skin. Due to its minimally invasive character, SMD can be applied in both clinical and preclinical settings. Despite being available since the 1990s, the technique has still not reached its full potential use as a tool to explore pathophysiological mechanisms of allergic and inflammatory reactions in the skin. Therefore, an EAACI Task Force on SMD was formed to disseminate knowledge about the technique and its many applications. This position paper from the task force provides an overview of the current use of SMD in the investigation of the pathogenesis of chronic inflammatory skin diseases, such as atopic dermatitis, chronic urticaria, psoriasis, and in studies of cutaneous events during type 1 hypersensitivity reactions. Furthermore, this paper covers drug hypersensitivity, UVB-induced- and neurogenic inflammation, and drug penetration investigated by SMD. The aim of this paper is to encourage the use of SMD and to make the technique easily accessible by providing an overview of methodology and applications, supported by standardized operating procedures for SMD in vivo and ex vivo.

AB - Skin microdialysis (SMD) is a versatile sampling technique that can be used to recover soluble endogenous and exogenous molecules from the extracellular compartment of human skin. Due to its minimally invasive character, SMD can be applied in both clinical and preclinical settings. Despite being available since the 1990s, the technique has still not reached its full potential use as a tool to explore pathophysiological mechanisms of allergic and inflammatory reactions in the skin. Therefore, an EAACI Task Force on SMD was formed to disseminate knowledge about the technique and its many applications. This position paper from the task force provides an overview of the current use of SMD in the investigation of the pathogenesis of chronic inflammatory skin diseases, such as atopic dermatitis, chronic urticaria, psoriasis, and in studies of cutaneous events during type 1 hypersensitivity reactions. Furthermore, this paper covers drug hypersensitivity, UVB-induced- and neurogenic inflammation, and drug penetration investigated by SMD. The aim of this paper is to encourage the use of SMD and to make the technique easily accessible by providing an overview of methodology and applications, supported by standardized operating procedures for SMD in vivo and ex vivo.

KW - Cutaneous

KW - Dermal

KW - Inflammation

KW - Interstitial

KW - Microdialysis

U2 - 10.1186/s13601-019-0262-y

DO - 10.1186/s13601-019-0262-y

M3 - Review

C2 - 31007896

AN - SCOPUS:85064214948

VL - 9

JO - Clinical and Translational Allergy

JF - Clinical and Translational Allergy

SN - 2045-7022

IS - 1

M1 - 24

ER -