Single-Cell Analysis Reveals Major Histocompatibility Complex II‒Expressing Keratinocytes in Pressure Ulcers with Worse Healing Outcomes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Single-Cell Analysis Reveals Major Histocompatibility Complex II‒Expressing Keratinocytes in Pressure Ulcers with Worse Healing Outcomes. / Li, Dongqing; Cheng, Shangli; Pei, Yu; Sommar, Pehr; Kärner, Jaanika; Herter, Eva K.; Toma, Maria A.; Zhang, Letian; Pham, Kim; Cheung, Yuen Ting; Liu, Zhuang; Chen, Xingqi; Eidsmo, Liv; Deng, Qiaolin; Xu Landén, Ning.

In: Journal of Investigative Dermatology, Vol. 142, No. 3, Part A, 2022, p. 705-716.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Li, D, Cheng, S, Pei, Y, Sommar, P, Kärner, J, Herter, EK, Toma, MA, Zhang, L, Pham, K, Cheung, YT, Liu, Z, Chen, X, Eidsmo, L, Deng, Q & Xu Landén, N 2022, 'Single-Cell Analysis Reveals Major Histocompatibility Complex II‒Expressing Keratinocytes in Pressure Ulcers with Worse Healing Outcomes', Journal of Investigative Dermatology, vol. 142, no. 3, Part A, pp. 705-716. https://doi.org/10.1016/j.jid.2021.07.176

APA

Li, D., Cheng, S., Pei, Y., Sommar, P., Kärner, J., Herter, E. K., Toma, M. A., Zhang, L., Pham, K., Cheung, Y. T., Liu, Z., Chen, X., Eidsmo, L., Deng, Q., & Xu Landén, N. (2022). Single-Cell Analysis Reveals Major Histocompatibility Complex II‒Expressing Keratinocytes in Pressure Ulcers with Worse Healing Outcomes. Journal of Investigative Dermatology, 142(3, Part A), 705-716. https://doi.org/10.1016/j.jid.2021.07.176

Vancouver

Li D, Cheng S, Pei Y, Sommar P, Kärner J, Herter EK et al. Single-Cell Analysis Reveals Major Histocompatibility Complex II‒Expressing Keratinocytes in Pressure Ulcers with Worse Healing Outcomes. Journal of Investigative Dermatology. 2022;142(3, Part A):705-716. https://doi.org/10.1016/j.jid.2021.07.176

Author

Li, Dongqing ; Cheng, Shangli ; Pei, Yu ; Sommar, Pehr ; Kärner, Jaanika ; Herter, Eva K. ; Toma, Maria A. ; Zhang, Letian ; Pham, Kim ; Cheung, Yuen Ting ; Liu, Zhuang ; Chen, Xingqi ; Eidsmo, Liv ; Deng, Qiaolin ; Xu Landén, Ning. / Single-Cell Analysis Reveals Major Histocompatibility Complex II‒Expressing Keratinocytes in Pressure Ulcers with Worse Healing Outcomes. In: Journal of Investigative Dermatology. 2022 ; Vol. 142, No. 3, Part A. pp. 705-716.

Bibtex

@article{23ed5126c3754a1da3e0d7f5970a55d6,
title = "Single-Cell Analysis Reveals Major Histocompatibility Complex II‒Expressing Keratinocytes in Pressure Ulcers with Worse Healing Outcomes",
abstract = "Pressure ulcer (PU) is a chronic wound often seen in patients with spinal cord injury and other bed-bound individuals, particularly in the elderly population. Despite its association with high mortality, the pathophysiology of PU remains poorly understood. In this study, we compared single-cell transcriptomic profiles of human epidermal cells from PU wound edges with those from uninjured skin and acute wounds in healthy donors. We identified significant shifts in the cell composition and gene expression patterns in PU. In particular, we found that major histocompatibility complex class II‒expressing keratinocytes were enriched in patients with worse healing outcomes. Furthermore, we showed that the IFN-γ in PU-derived wound fluid could induce major histocompatibility complex II expression in keratinocytes and that these wound fluid‒treated keratinocytes inhibited autologous T-cell activation. In line with this observation, we found that T cells from PUs enriched with major histocompatibility complex II+ keratinocytes produced fewer inflammatory cytokines. Overall, our study provides a high-resolution molecular map of human PU compared with that of acute wounds and intact skin, providing insights into PU pathology and the future development of tailored wound therapy.",
author = "Dongqing Li and Shangli Cheng and Yu Pei and Pehr Sommar and Jaanika K{\"a}rner and Herter, {Eva K.} and Toma, {Maria A.} and Letian Zhang and Kim Pham and Cheung, {Yuen Ting} and Zhuang Liu and Xingqi Chen and Liv Eidsmo and Qiaolin Deng and {Xu Land{\'e}n}, Ning",
note = "Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2022",
doi = "10.1016/j.jid.2021.07.176",
language = "English",
volume = "142",
pages = "705--716",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "nature publishing group",
number = "3, Part A",

}

RIS

TY - JOUR

T1 - Single-Cell Analysis Reveals Major Histocompatibility Complex II‒Expressing Keratinocytes in Pressure Ulcers with Worse Healing Outcomes

AU - Li, Dongqing

AU - Cheng, Shangli

AU - Pei, Yu

AU - Sommar, Pehr

AU - Kärner, Jaanika

AU - Herter, Eva K.

AU - Toma, Maria A.

AU - Zhang, Letian

AU - Pham, Kim

AU - Cheung, Yuen Ting

AU - Liu, Zhuang

AU - Chen, Xingqi

AU - Eidsmo, Liv

AU - Deng, Qiaolin

AU - Xu Landén, Ning

N1 - Publisher Copyright: © 2021 The Authors

PY - 2022

Y1 - 2022

N2 - Pressure ulcer (PU) is a chronic wound often seen in patients with spinal cord injury and other bed-bound individuals, particularly in the elderly population. Despite its association with high mortality, the pathophysiology of PU remains poorly understood. In this study, we compared single-cell transcriptomic profiles of human epidermal cells from PU wound edges with those from uninjured skin and acute wounds in healthy donors. We identified significant shifts in the cell composition and gene expression patterns in PU. In particular, we found that major histocompatibility complex class II‒expressing keratinocytes were enriched in patients with worse healing outcomes. Furthermore, we showed that the IFN-γ in PU-derived wound fluid could induce major histocompatibility complex II expression in keratinocytes and that these wound fluid‒treated keratinocytes inhibited autologous T-cell activation. In line with this observation, we found that T cells from PUs enriched with major histocompatibility complex II+ keratinocytes produced fewer inflammatory cytokines. Overall, our study provides a high-resolution molecular map of human PU compared with that of acute wounds and intact skin, providing insights into PU pathology and the future development of tailored wound therapy.

AB - Pressure ulcer (PU) is a chronic wound often seen in patients with spinal cord injury and other bed-bound individuals, particularly in the elderly population. Despite its association with high mortality, the pathophysiology of PU remains poorly understood. In this study, we compared single-cell transcriptomic profiles of human epidermal cells from PU wound edges with those from uninjured skin and acute wounds in healthy donors. We identified significant shifts in the cell composition and gene expression patterns in PU. In particular, we found that major histocompatibility complex class II‒expressing keratinocytes were enriched in patients with worse healing outcomes. Furthermore, we showed that the IFN-γ in PU-derived wound fluid could induce major histocompatibility complex II expression in keratinocytes and that these wound fluid‒treated keratinocytes inhibited autologous T-cell activation. In line with this observation, we found that T cells from PUs enriched with major histocompatibility complex II+ keratinocytes produced fewer inflammatory cytokines. Overall, our study provides a high-resolution molecular map of human PU compared with that of acute wounds and intact skin, providing insights into PU pathology and the future development of tailored wound therapy.

U2 - 10.1016/j.jid.2021.07.176

DO - 10.1016/j.jid.2021.07.176

M3 - Journal article

C2 - 34536485

AN - SCOPUS:85116880262

VL - 142

SP - 705

EP - 716

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 3, Part A

ER -

ID: 283139463