SHP2 regulates IL-2 induced MAPK activation, but not Stat3 or Stat5 tyrosine phosphorylation, in cutaneous T cell lymphoma cells

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SHP2 regulates IL-2 induced MAPK activation, but not Stat3 or Stat5 tyrosine phosphorylation, in cutaneous T cell lymphoma cells. / Lundin Brockdorff, Johannes; Woetmann, Anders; Mustelin, Tomas; Kaltoft, Keld; Zhang, Qian; Wasik, Mariusz A; Röpke, Carsten; Ødum, Niels.

In: Cytokine, Vol. 20, No. 4, 2002, p. 141-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lundin Brockdorff, J, Woetmann, A, Mustelin, T, Kaltoft, K, Zhang, Q, Wasik, MA, Röpke, C & Ødum, N 2002, 'SHP2 regulates IL-2 induced MAPK activation, but not Stat3 or Stat5 tyrosine phosphorylation, in cutaneous T cell lymphoma cells', Cytokine, vol. 20, no. 4, pp. 141-7. https://doi.org/10.1006/cyto.2002.1986

APA

Lundin Brockdorff, J., Woetmann, A., Mustelin, T., Kaltoft, K., Zhang, Q., Wasik, M. A., Röpke, C., & Ødum, N. (2002). SHP2 regulates IL-2 induced MAPK activation, but not Stat3 or Stat5 tyrosine phosphorylation, in cutaneous T cell lymphoma cells. Cytokine, 20(4), 141-7. https://doi.org/10.1006/cyto.2002.1986

Vancouver

Lundin Brockdorff J, Woetmann A, Mustelin T, Kaltoft K, Zhang Q, Wasik MA et al. SHP2 regulates IL-2 induced MAPK activation, but not Stat3 or Stat5 tyrosine phosphorylation, in cutaneous T cell lymphoma cells. Cytokine. 2002;20(4):141-7. https://doi.org/10.1006/cyto.2002.1986

Author

Lundin Brockdorff, Johannes ; Woetmann, Anders ; Mustelin, Tomas ; Kaltoft, Keld ; Zhang, Qian ; Wasik, Mariusz A ; Röpke, Carsten ; Ødum, Niels. / SHP2 regulates IL-2 induced MAPK activation, but not Stat3 or Stat5 tyrosine phosphorylation, in cutaneous T cell lymphoma cells. In: Cytokine. 2002 ; Vol. 20, No. 4. pp. 141-7.

Bibtex

@article{538b1f80fcf011ddb219000ea68e967b,
title = "SHP2 regulates IL-2 induced MAPK activation, but not Stat3 or Stat5 tyrosine phosphorylation, in cutaneous T cell lymphoma cells",
abstract = "The phosphotyrosine phosphatase SHP2 has been suggested to regulate activation of MAPK, Stat3, and Stat5 in several experimental models. In this study we investigated the role of SHP2 in IL-2 induced activation of MAPK and the Stat proteins using the human CTCL cell line MyLa2059 derived from a cutaneous T cell lymphoma (CTCL). For this purpose, MyLa2059 cells were stably transfected with wild-type SHP2 or inactive SHP2. The cells transfected with inactive SHP2 showed reduced MAPK activation upon IL-2 stimulation, suggesting that SHP2 upregulates IL-2 induced MAPK activation in T cells. However, the constitutive tyrosine phosphorylation of Stat3 as well as IL-2 induced Stat5 tyrosine phosphorylation and DNA binding were unaffected by the stably transfected wild-type SHP2 as well as the inactive SHP2. In conclusion, we show for the first time that SHP2 positively regulates IL-2 induced MAPK activation in malignant T cells. Furthermore, the results indicate that SHP2 may not be involved in the activation of Stat3 or Stat5 in CTCL cells.",
author = "{Lundin Brockdorff}, Johannes and Anders Woetmann and Tomas Mustelin and Keld Kaltoft and Qian Zhang and Wasik, {Mariusz A} and Carsten R{\"o}pke and Niels {\O}dum",
note = "Keywords: Amino Acid Substitution; DNA, Neoplasm; DNA-Binding Proteins; Enzyme Activation; Humans; Interleukin-2; Intracellular Signaling Peptides and Proteins; MAP Kinase Kinase Kinase 1; MAP Kinase Signaling System; Milk Proteins; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases; Mutation, Missense; Mycosis Fungoides; Neoplasm Proteins; Phosphorylation; Protein Binding; Protein Processing, Post-Translational; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Protein Tyrosine Phosphatases; Protein-Serine-Threonine Kinases; Recombinant Fusion Proteins; STAT3 Transcription Factor; STAT5 Transcription Factor; Trans-Activators; Transfection; Tumor Cells, Cultured",
year = "2002",
doi = "10.1006/cyto.2002.1986",
language = "English",
volume = "20",
pages = "141--7",
journal = "Cytokine",
issn = "1043-4666",
publisher = "Academic Press",
number = "4",

}

RIS

TY - JOUR

T1 - SHP2 regulates IL-2 induced MAPK activation, but not Stat3 or Stat5 tyrosine phosphorylation, in cutaneous T cell lymphoma cells

AU - Lundin Brockdorff, Johannes

AU - Woetmann, Anders

AU - Mustelin, Tomas

AU - Kaltoft, Keld

AU - Zhang, Qian

AU - Wasik, Mariusz A

AU - Röpke, Carsten

AU - Ødum, Niels

N1 - Keywords: Amino Acid Substitution; DNA, Neoplasm; DNA-Binding Proteins; Enzyme Activation; Humans; Interleukin-2; Intracellular Signaling Peptides and Proteins; MAP Kinase Kinase Kinase 1; MAP Kinase Signaling System; Milk Proteins; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Mitogen-Activated Protein Kinases; Mutation, Missense; Mycosis Fungoides; Neoplasm Proteins; Phosphorylation; Protein Binding; Protein Processing, Post-Translational; Protein Tyrosine Phosphatase, Non-Receptor Type 11; Protein Tyrosine Phosphatases; Protein-Serine-Threonine Kinases; Recombinant Fusion Proteins; STAT3 Transcription Factor; STAT5 Transcription Factor; Trans-Activators; Transfection; Tumor Cells, Cultured

PY - 2002

Y1 - 2002

N2 - The phosphotyrosine phosphatase SHP2 has been suggested to regulate activation of MAPK, Stat3, and Stat5 in several experimental models. In this study we investigated the role of SHP2 in IL-2 induced activation of MAPK and the Stat proteins using the human CTCL cell line MyLa2059 derived from a cutaneous T cell lymphoma (CTCL). For this purpose, MyLa2059 cells were stably transfected with wild-type SHP2 or inactive SHP2. The cells transfected with inactive SHP2 showed reduced MAPK activation upon IL-2 stimulation, suggesting that SHP2 upregulates IL-2 induced MAPK activation in T cells. However, the constitutive tyrosine phosphorylation of Stat3 as well as IL-2 induced Stat5 tyrosine phosphorylation and DNA binding were unaffected by the stably transfected wild-type SHP2 as well as the inactive SHP2. In conclusion, we show for the first time that SHP2 positively regulates IL-2 induced MAPK activation in malignant T cells. Furthermore, the results indicate that SHP2 may not be involved in the activation of Stat3 or Stat5 in CTCL cells.

AB - The phosphotyrosine phosphatase SHP2 has been suggested to regulate activation of MAPK, Stat3, and Stat5 in several experimental models. In this study we investigated the role of SHP2 in IL-2 induced activation of MAPK and the Stat proteins using the human CTCL cell line MyLa2059 derived from a cutaneous T cell lymphoma (CTCL). For this purpose, MyLa2059 cells were stably transfected with wild-type SHP2 or inactive SHP2. The cells transfected with inactive SHP2 showed reduced MAPK activation upon IL-2 stimulation, suggesting that SHP2 upregulates IL-2 induced MAPK activation in T cells. However, the constitutive tyrosine phosphorylation of Stat3 as well as IL-2 induced Stat5 tyrosine phosphorylation and DNA binding were unaffected by the stably transfected wild-type SHP2 as well as the inactive SHP2. In conclusion, we show for the first time that SHP2 positively regulates IL-2 induced MAPK activation in malignant T cells. Furthermore, the results indicate that SHP2 may not be involved in the activation of Stat3 or Stat5 in CTCL cells.

U2 - 10.1006/cyto.2002.1986

DO - 10.1006/cyto.2002.1986

M3 - Journal article

C2 - 12543077

VL - 20

SP - 141

EP - 147

JO - Cytokine

JF - Cytokine

SN - 1043-4666

IS - 4

ER -

ID: 10615324