Midline 1 directs lytic granule exocytosis and cytotoxicity of mouse killer T cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Midline 1 directs lytic granule exocytosis and cytotoxicity of mouse killer T cells. / Boding, Lasse; Hansen, Ann K; Meroni, Germana; Johansen, Bo B; Braunstein, Thomas H; Bonefeld, Charlotte M; Kongsbak, Martin; Jensen, Benjamin A H; Woetmann, Anders; Thomsen, Allan Randrup; Odum, Niels; von Essen, Marina R; Geisler, Carsten.

In: European Journal of Immunology, Vol. 44, No. 10, 12.07.2014, p. 3109-3118.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Boding, L, Hansen, AK, Meroni, G, Johansen, BB, Braunstein, TH, Bonefeld, CM, Kongsbak, M, Jensen, BAH, Woetmann, A, Thomsen, AR, Odum, N, von Essen, MR & Geisler, C 2014, 'Midline 1 directs lytic granule exocytosis and cytotoxicity of mouse killer T cells', European Journal of Immunology, vol. 44, no. 10, pp. 3109-3118. https://doi.org/10.1002/eji.201344388

APA

Boding, L., Hansen, A. K., Meroni, G., Johansen, B. B., Braunstein, T. H., Bonefeld, C. M., Kongsbak, M., Jensen, B. A. H., Woetmann, A., Thomsen, A. R., Odum, N., von Essen, M. R., & Geisler, C. (2014). Midline 1 directs lytic granule exocytosis and cytotoxicity of mouse killer T cells. European Journal of Immunology, 44(10), 3109-3118. https://doi.org/10.1002/eji.201344388

Vancouver

Boding L, Hansen AK, Meroni G, Johansen BB, Braunstein TH, Bonefeld CM et al. Midline 1 directs lytic granule exocytosis and cytotoxicity of mouse killer T cells. European Journal of Immunology. 2014 Jul 12;44(10):3109-3118. https://doi.org/10.1002/eji.201344388

Author

Boding, Lasse ; Hansen, Ann K ; Meroni, Germana ; Johansen, Bo B ; Braunstein, Thomas H ; Bonefeld, Charlotte M ; Kongsbak, Martin ; Jensen, Benjamin A H ; Woetmann, Anders ; Thomsen, Allan Randrup ; Odum, Niels ; von Essen, Marina R ; Geisler, Carsten. / Midline 1 directs lytic granule exocytosis and cytotoxicity of mouse killer T cells. In: European Journal of Immunology. 2014 ; Vol. 44, No. 10. pp. 3109-3118.

Bibtex

@article{ffd0ecdece0e4d4f882475f31946d648,
title = "Midline 1 directs lytic granule exocytosis and cytotoxicity of mouse killer T cells",
abstract = "Midline 1 (MID1) is a microtubule-associated ubiquitin ligase that regulates protein phosphatase 2A activity. Loss-of-function mutations in MID1 lead to the X-linked Opitz G/BBB syndrome characterized by defective midline development during embryogenesis. Here we show that MID1 is strongly upregulated in murine cytotoxic lymphocytes (CTLs), and that it controls TCR signaling, centrosome trafficking and exocytosis of lytic granules. In accordance, we find that the killing capacity of MID1(-/-) CTLs is impaired. Transfection of MID1 into MID1(-/-) CTLs completely rescued lytic granule exocytosis, and vice versa, knock-down of MID1 inhibited exocytosis of lytic granules in wild-type CTLs, cementing a central role for MID1 in the regulation of granule exocytosis. Thus, MID1 orchestrates multiple events in CTL responses, adding a novel level of regulation to CTL activation and cytotoxicity. This article is protected by copyright. All rights reserved.",
author = "Lasse Boding and Hansen, {Ann K} and Germana Meroni and Johansen, {Bo B} and Braunstein, {Thomas H} and Bonefeld, {Charlotte M} and Martin Kongsbak and Jensen, {Benjamin A H} and Anders Woetmann and Thomsen, {Allan Randrup} and Niels Odum and {von Essen}, {Marina R} and Carsten Geisler",
note = "This article is protected by copyright. All rights reserved.",
year = "2014",
month = jul,
day = "12",
doi = "10.1002/eji.201344388",
language = "English",
volume = "44",
pages = "3109--3118",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "10",

}

RIS

TY - JOUR

T1 - Midline 1 directs lytic granule exocytosis and cytotoxicity of mouse killer T cells

AU - Boding, Lasse

AU - Hansen, Ann K

AU - Meroni, Germana

AU - Johansen, Bo B

AU - Braunstein, Thomas H

AU - Bonefeld, Charlotte M

AU - Kongsbak, Martin

AU - Jensen, Benjamin A H

AU - Woetmann, Anders

AU - Thomsen, Allan Randrup

AU - Odum, Niels

AU - von Essen, Marina R

AU - Geisler, Carsten

N1 - This article is protected by copyright. All rights reserved.

PY - 2014/7/12

Y1 - 2014/7/12

N2 - Midline 1 (MID1) is a microtubule-associated ubiquitin ligase that regulates protein phosphatase 2A activity. Loss-of-function mutations in MID1 lead to the X-linked Opitz G/BBB syndrome characterized by defective midline development during embryogenesis. Here we show that MID1 is strongly upregulated in murine cytotoxic lymphocytes (CTLs), and that it controls TCR signaling, centrosome trafficking and exocytosis of lytic granules. In accordance, we find that the killing capacity of MID1(-/-) CTLs is impaired. Transfection of MID1 into MID1(-/-) CTLs completely rescued lytic granule exocytosis, and vice versa, knock-down of MID1 inhibited exocytosis of lytic granules in wild-type CTLs, cementing a central role for MID1 in the regulation of granule exocytosis. Thus, MID1 orchestrates multiple events in CTL responses, adding a novel level of regulation to CTL activation and cytotoxicity. This article is protected by copyright. All rights reserved.

AB - Midline 1 (MID1) is a microtubule-associated ubiquitin ligase that regulates protein phosphatase 2A activity. Loss-of-function mutations in MID1 lead to the X-linked Opitz G/BBB syndrome characterized by defective midline development during embryogenesis. Here we show that MID1 is strongly upregulated in murine cytotoxic lymphocytes (CTLs), and that it controls TCR signaling, centrosome trafficking and exocytosis of lytic granules. In accordance, we find that the killing capacity of MID1(-/-) CTLs is impaired. Transfection of MID1 into MID1(-/-) CTLs completely rescued lytic granule exocytosis, and vice versa, knock-down of MID1 inhibited exocytosis of lytic granules in wild-type CTLs, cementing a central role for MID1 in the regulation of granule exocytosis. Thus, MID1 orchestrates multiple events in CTL responses, adding a novel level of regulation to CTL activation and cytotoxicity. This article is protected by copyright. All rights reserved.

U2 - 10.1002/eji.201344388

DO - 10.1002/eji.201344388

M3 - Journal article

C2 - 25043946

VL - 44

SP - 3109

EP - 3118

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 10

ER -

ID: 119526093