MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells

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Standard

MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells. / Boding, Lasse; Hansen, Ann K; Meroni, Germana; Levring, Trine B; Andersen, Anders Woetmann; Ødum, Niels; Bonefeld, Charlotte M; Geisler, Carsten.

In: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 123, No. 8, 08.2015, p. 682-7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Boding, L, Hansen, AK, Meroni, G, Levring, TB, Andersen, AW, Ødum, N, Bonefeld, CM & Geisler, C 2015, 'MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells', APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, vol. 123, no. 8, pp. 682-7. https://doi.org/10.1111/apm.12402

APA

Boding, L., Hansen, A. K., Meroni, G., Levring, T. B., Andersen, A. W., Ødum, N., Bonefeld, C. M., & Geisler, C. (2015). MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 123(8), 682-7. https://doi.org/10.1111/apm.12402

Vancouver

Boding L, Hansen AK, Meroni G, Levring TB, Andersen AW, Ødum N et al. MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2015 Aug;123(8):682-7. https://doi.org/10.1111/apm.12402

Author

Boding, Lasse ; Hansen, Ann K ; Meroni, Germana ; Levring, Trine B ; Andersen, Anders Woetmann ; Ødum, Niels ; Bonefeld, Charlotte M ; Geisler, Carsten. / MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells. In: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2015 ; Vol. 123, No. 8. pp. 682-7.

Bibtex

@article{f18a7cd3c4e643659496c2ad058144aa,
title = "MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells",
abstract = "We have recently shown that the E3 ubiquitin ligase midline 1 (MID1) is upregulated in murine cytotoxic lymphocytes (CTL), where it controls exocytosis of lytic granules and the killing capacity. Accordingly, CTL from MID1 knock-out (MID1(-/-)) mice have a 25-30% reduction in exocytosis of lytic granules and cytotoxicity compared to CTL from wild-type (WT) mice. We wondered why the MID1 gene knock-out did not affect exocytosis and cytotoxicity more severely and speculated whether MID2, a close homologue of MID1, might partially compensate for the loss of MID1 in MID1(-/-) CTL. Here, we showed that MID2, like MID1, is upregulated in activated murine T cells. Furthermore, MID1(-/-) CTL upregulated MID2 two-twenty-fold stronger than CTL from WT mice, suggesting that MID2 might compensate for MID1. In agreement, transfection of MID2 into MID1(-/-) CTL completely rescued exocytosis of lytic granules in MID1(-/-) CTL, and vice versa, knock-down of MID2 inhibited exocytosis of lytic granules in both WT and MID1(-/-) CTL, demonstrating that both MID1 and MID2 play a central role in the regulation of granule exocytosis and that functional redundancy exists between MID1 and MID2 in CTL.",
keywords = "Animals, Cytoplasmic Granules, Exocytosis, Interferon-gamma, Mice, Mice, Inbred C57BL, Mice, Knockout, Microtubule-Associated Proteins, Proteins, T-Lymphocytes, Cytotoxic, Transcription Factors, Up-Regulation",
author = "Lasse Boding and Hansen, {Ann K} and Germana Meroni and Levring, {Trine B} and Andersen, {Anders Woetmann} and Niels {\O}dum and Bonefeld, {Charlotte M} and Carsten Geisler",
note = "{\textcopyright} 2015 APMIS. Published by John Wiley & Sons Ltd.",
year = "2015",
month = aug,
doi = "10.1111/apm.12402",
language = "English",
volume = "123",
pages = "682--7",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "8",

}

RIS

TY - JOUR

T1 - MID2 can substitute for MID1 and control exocytosis of lytic granules in cytotoxic T cells

AU - Boding, Lasse

AU - Hansen, Ann K

AU - Meroni, Germana

AU - Levring, Trine B

AU - Andersen, Anders Woetmann

AU - Ødum, Niels

AU - Bonefeld, Charlotte M

AU - Geisler, Carsten

N1 - © 2015 APMIS. Published by John Wiley & Sons Ltd.

PY - 2015/8

Y1 - 2015/8

N2 - We have recently shown that the E3 ubiquitin ligase midline 1 (MID1) is upregulated in murine cytotoxic lymphocytes (CTL), where it controls exocytosis of lytic granules and the killing capacity. Accordingly, CTL from MID1 knock-out (MID1(-/-)) mice have a 25-30% reduction in exocytosis of lytic granules and cytotoxicity compared to CTL from wild-type (WT) mice. We wondered why the MID1 gene knock-out did not affect exocytosis and cytotoxicity more severely and speculated whether MID2, a close homologue of MID1, might partially compensate for the loss of MID1 in MID1(-/-) CTL. Here, we showed that MID2, like MID1, is upregulated in activated murine T cells. Furthermore, MID1(-/-) CTL upregulated MID2 two-twenty-fold stronger than CTL from WT mice, suggesting that MID2 might compensate for MID1. In agreement, transfection of MID2 into MID1(-/-) CTL completely rescued exocytosis of lytic granules in MID1(-/-) CTL, and vice versa, knock-down of MID2 inhibited exocytosis of lytic granules in both WT and MID1(-/-) CTL, demonstrating that both MID1 and MID2 play a central role in the regulation of granule exocytosis and that functional redundancy exists between MID1 and MID2 in CTL.

AB - We have recently shown that the E3 ubiquitin ligase midline 1 (MID1) is upregulated in murine cytotoxic lymphocytes (CTL), where it controls exocytosis of lytic granules and the killing capacity. Accordingly, CTL from MID1 knock-out (MID1(-/-)) mice have a 25-30% reduction in exocytosis of lytic granules and cytotoxicity compared to CTL from wild-type (WT) mice. We wondered why the MID1 gene knock-out did not affect exocytosis and cytotoxicity more severely and speculated whether MID2, a close homologue of MID1, might partially compensate for the loss of MID1 in MID1(-/-) CTL. Here, we showed that MID2, like MID1, is upregulated in activated murine T cells. Furthermore, MID1(-/-) CTL upregulated MID2 two-twenty-fold stronger than CTL from WT mice, suggesting that MID2 might compensate for MID1. In agreement, transfection of MID2 into MID1(-/-) CTL completely rescued exocytosis of lytic granules in MID1(-/-) CTL, and vice versa, knock-down of MID2 inhibited exocytosis of lytic granules in both WT and MID1(-/-) CTL, demonstrating that both MID1 and MID2 play a central role in the regulation of granule exocytosis and that functional redundancy exists between MID1 and MID2 in CTL.

KW - Animals

KW - Cytoplasmic Granules

KW - Exocytosis

KW - Interferon-gamma

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Microtubule-Associated Proteins

KW - Proteins

KW - T-Lymphocytes, Cytotoxic

KW - Transcription Factors

KW - Up-Regulation

U2 - 10.1111/apm.12402

DO - 10.1111/apm.12402

M3 - Journal article

C2 - 25924778

VL - 123

SP - 682

EP - 687

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 8

ER -

ID: 158578325