In vitro differentiated human CD4+ T cells produce hepatocyte growth factor

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

In vitro differentiated human CD4+ T cells produce hepatocyte growth factor. / Ford, Shayne Lavondua; Buus, Terkild Brink; Nastasi, Claudia; Geisler, Carsten; Bonefeld, Charlotte Menné; Ødum, Niels; Woetmann, Anders.

In: Frontiers in Immunology, Vol. 14, 1210836, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ford, SL, Buus, TB, Nastasi, C, Geisler, C, Bonefeld, CM, Ødum, N & Woetmann, A 2023, 'In vitro differentiated human CD4+ T cells produce hepatocyte growth factor', Frontiers in Immunology, vol. 14, 1210836. https://doi.org/10.3389/fimmu.2023.1210836

APA

Ford, S. L., Buus, T. B., Nastasi, C., Geisler, C., Bonefeld, C. M., Ødum, N., & Woetmann, A. (2023). In vitro differentiated human CD4+ T cells produce hepatocyte growth factor. Frontiers in Immunology, 14, [1210836]. https://doi.org/10.3389/fimmu.2023.1210836

Vancouver

Ford SL, Buus TB, Nastasi C, Geisler C, Bonefeld CM, Ødum N et al. In vitro differentiated human CD4+ T cells produce hepatocyte growth factor. Frontiers in Immunology. 2023;14. 1210836. https://doi.org/10.3389/fimmu.2023.1210836

Author

Ford, Shayne Lavondua ; Buus, Terkild Brink ; Nastasi, Claudia ; Geisler, Carsten ; Bonefeld, Charlotte Menné ; Ødum, Niels ; Woetmann, Anders. / In vitro differentiated human CD4+ T cells produce hepatocyte growth factor. In: Frontiers in Immunology. 2023 ; Vol. 14.

Bibtex

@article{742af1c53c0f4e9e9b82d0cf32209743,
title = "In vitro differentiated human CD4+ T cells produce hepatocyte growth factor",
abstract = "Differentiation of naive CD4+ T cells into effector T cells is a dynamic process in which the cells are polarized into T helper (Th) subsets. The subsets largely consist of four fundamental categories: Th1, Th2, Th17, and regulatory T cells. We show that human memory CD4+ T cells can produce hepatocyte growth factor (HGF), a pleiotropic cytokine which can affect several tissue types through signaling by its receptor, c-Met. In vitro differentiation of T cells into Th-like subsets revealed that HGF producing T cells increase under Th1 conditions. Enrichment of HGF producing cells was possible by targeting cells with surface CD30 expression, a marker discovered through single-cell RNA-sequencing. Furthermore, pharmacological inhibition of PI3K or mTOR was found to inhibit HGF mRNA and protein, while an Akt inhibitor was found to increase these levels. The findings suggest that HGF producing T cells could play a role in disease where Th1 are present.",
keywords = "c-Met, cytokine, growth factor, hepatocyte growth factor, HGF, sequencing, T cell",
author = "Ford, {Shayne Lavondua} and Buus, {Terkild Brink} and Claudia Nastasi and Carsten Geisler and Bonefeld, {Charlotte Menn{\'e}} and Niels {\O}dum and Anders Woetmann",
note = "Publisher Copyright: Copyright {\textcopyright} 2023 Ford, Buus, Nastasi, Geisler, Bonefeld, {\O}dum and Woetmann.",
year = "2023",
doi = "10.3389/fimmu.2023.1210836",
language = "English",
volume = "14",
journal = "Frontiers in Immunology",
issn = "1664-3224",
publisher = "Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - In vitro differentiated human CD4+ T cells produce hepatocyte growth factor

AU - Ford, Shayne Lavondua

AU - Buus, Terkild Brink

AU - Nastasi, Claudia

AU - Geisler, Carsten

AU - Bonefeld, Charlotte Menné

AU - Ødum, Niels

AU - Woetmann, Anders

N1 - Publisher Copyright: Copyright © 2023 Ford, Buus, Nastasi, Geisler, Bonefeld, Ødum and Woetmann.

PY - 2023

Y1 - 2023

N2 - Differentiation of naive CD4+ T cells into effector T cells is a dynamic process in which the cells are polarized into T helper (Th) subsets. The subsets largely consist of four fundamental categories: Th1, Th2, Th17, and regulatory T cells. We show that human memory CD4+ T cells can produce hepatocyte growth factor (HGF), a pleiotropic cytokine which can affect several tissue types through signaling by its receptor, c-Met. In vitro differentiation of T cells into Th-like subsets revealed that HGF producing T cells increase under Th1 conditions. Enrichment of HGF producing cells was possible by targeting cells with surface CD30 expression, a marker discovered through single-cell RNA-sequencing. Furthermore, pharmacological inhibition of PI3K or mTOR was found to inhibit HGF mRNA and protein, while an Akt inhibitor was found to increase these levels. The findings suggest that HGF producing T cells could play a role in disease where Th1 are present.

AB - Differentiation of naive CD4+ T cells into effector T cells is a dynamic process in which the cells are polarized into T helper (Th) subsets. The subsets largely consist of four fundamental categories: Th1, Th2, Th17, and regulatory T cells. We show that human memory CD4+ T cells can produce hepatocyte growth factor (HGF), a pleiotropic cytokine which can affect several tissue types through signaling by its receptor, c-Met. In vitro differentiation of T cells into Th-like subsets revealed that HGF producing T cells increase under Th1 conditions. Enrichment of HGF producing cells was possible by targeting cells with surface CD30 expression, a marker discovered through single-cell RNA-sequencing. Furthermore, pharmacological inhibition of PI3K or mTOR was found to inhibit HGF mRNA and protein, while an Akt inhibitor was found to increase these levels. The findings suggest that HGF producing T cells could play a role in disease where Th1 are present.

KW - c-Met

KW - cytokine

KW - growth factor

KW - hepatocyte growth factor

KW - HGF

KW - sequencing

KW - T cell

U2 - 10.3389/fimmu.2023.1210836

DO - 10.3389/fimmu.2023.1210836

M3 - Journal article

C2 - 37520551

AN - SCOPUS:85165939248

VL - 14

JO - Frontiers in Immunology

JF - Frontiers in Immunology

SN - 1664-3224

M1 - 1210836

ER -

ID: 362696182