IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes : Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. / Padhi, Avinash; Srivastava, Ankit; Ramesh, Abarajitha; Ehrström, Marcus; Simon, Michel; Sonkoly, Enikö; Eidsmo, Liv; Bergman, Peter; Lysell, Josefin.

In: The Journal of Investigative Dermatology, Vol. 142, No. 2, 2022, p. 333-342.e6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Padhi, A, Srivastava, A, Ramesh, A, Ehrström, M, Simon, M, Sonkoly, E, Eidsmo, L, Bergman, P & Lysell, J 2022, 'IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation', The Journal of Investigative Dermatology, vol. 142, no. 2, pp. 333-342.e6. https://doi.org/10.1016/j.jid.2021.07.155

APA

Padhi, A., Srivastava, A., Ramesh, A., Ehrström, M., Simon, M., Sonkoly, E., Eidsmo, L., Bergman, P., & Lysell, J. (2022). IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. The Journal of Investigative Dermatology, 142(2), 333-342.e6. https://doi.org/10.1016/j.jid.2021.07.155

Vancouver

Padhi A, Srivastava A, Ramesh A, Ehrström M, Simon M, Sonkoly E et al. IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. The Journal of Investigative Dermatology. 2022;142(2):333-342.e6. https://doi.org/10.1016/j.jid.2021.07.155

Author

Padhi, Avinash ; Srivastava, Ankit ; Ramesh, Abarajitha ; Ehrström, Marcus ; Simon, Michel ; Sonkoly, Enikö ; Eidsmo, Liv ; Bergman, Peter ; Lysell, Josefin. / IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes : Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation. In: The Journal of Investigative Dermatology. 2022 ; Vol. 142, No. 2. pp. 333-342.e6.

Bibtex

@article{367ee01a27f54b78ba78966b2ee3b60e,
title = "IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes: Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation",
abstract = "Increased presence of IL-22+ cells in the skin is a characteristic finding in skin barrier defects, such as psoriasis and atopic dermatitis. However, mechanistic insight into effects of IL-22 on epidermal functioning is yet to be elucidated. One crucial step during epidermal differentiation is deimination or citrullination. Here, we show reduced levels of peptidylarginine deiminase 1, an enzyme that converts peptidylarginine into citrulline in lesional psoriatic skin. IL-22 signaling through the IL-22 receptor complex was found to suppress expression of peptidylarginine deiminase 1 in epidermal keratinocytes. Subsequently, total peptidylarginine deiminase activity and extent of protein deimination in keratinocytes treated with IL-22 were reduced together with a significant decrease in deimination of keratin 1 and FLG, both important for epidermal differentiation. Vitamin D and acitretin partly restored the peptidylarginine deiminase 1 defect caused by IL-22. Collectively, we show that IL-22 downregulates deimination, thus identifying a potential target for treatment of skin barrier defects.",
keywords = "Acitretin/pharmacology, Biopsy, Cell Differentiation/drug effects, Cell Line, Citrullination/drug effects, Dermatitis, Atopic/drug therapy, Down-Regulation, Epidermis/drug effects, Filaggrin Proteins/metabolism, Humans, Interleukins/metabolism, Keratin-1/metabolism, Keratinocytes/drug effects, Primary Cell Culture, Protein-Arginine Deiminase Type 1/genetics, Psoriasis/drug therapy, Vitamin D/pharmacology",
author = "Avinash Padhi and Ankit Srivastava and Abarajitha Ramesh and Marcus Ehrstr{\"o}m and Michel Simon and Enik{\"o} Sonkoly and Liv Eidsmo and Peter Bergman and Josefin Lysell",
note = "Copyright {\textcopyright} 2021 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2022",
doi = "10.1016/j.jid.2021.07.155",
language = "English",
volume = "142",
pages = "333--342.e6",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "nature publishing group",
number = "2",

}

RIS

TY - JOUR

T1 - IL-22 Downregulates Peptidylarginine Deiminase-1 in Human Keratinocytes

T2 - Adding Another Piece to the IL-22 Puzzle in Epidermal Barrier Formation

AU - Padhi, Avinash

AU - Srivastava, Ankit

AU - Ramesh, Abarajitha

AU - Ehrström, Marcus

AU - Simon, Michel

AU - Sonkoly, Enikö

AU - Eidsmo, Liv

AU - Bergman, Peter

AU - Lysell, Josefin

N1 - Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2022

Y1 - 2022

N2 - Increased presence of IL-22+ cells in the skin is a characteristic finding in skin barrier defects, such as psoriasis and atopic dermatitis. However, mechanistic insight into effects of IL-22 on epidermal functioning is yet to be elucidated. One crucial step during epidermal differentiation is deimination or citrullination. Here, we show reduced levels of peptidylarginine deiminase 1, an enzyme that converts peptidylarginine into citrulline in lesional psoriatic skin. IL-22 signaling through the IL-22 receptor complex was found to suppress expression of peptidylarginine deiminase 1 in epidermal keratinocytes. Subsequently, total peptidylarginine deiminase activity and extent of protein deimination in keratinocytes treated with IL-22 were reduced together with a significant decrease in deimination of keratin 1 and FLG, both important for epidermal differentiation. Vitamin D and acitretin partly restored the peptidylarginine deiminase 1 defect caused by IL-22. Collectively, we show that IL-22 downregulates deimination, thus identifying a potential target for treatment of skin barrier defects.

AB - Increased presence of IL-22+ cells in the skin is a characteristic finding in skin barrier defects, such as psoriasis and atopic dermatitis. However, mechanistic insight into effects of IL-22 on epidermal functioning is yet to be elucidated. One crucial step during epidermal differentiation is deimination or citrullination. Here, we show reduced levels of peptidylarginine deiminase 1, an enzyme that converts peptidylarginine into citrulline in lesional psoriatic skin. IL-22 signaling through the IL-22 receptor complex was found to suppress expression of peptidylarginine deiminase 1 in epidermal keratinocytes. Subsequently, total peptidylarginine deiminase activity and extent of protein deimination in keratinocytes treated with IL-22 were reduced together with a significant decrease in deimination of keratin 1 and FLG, both important for epidermal differentiation. Vitamin D and acitretin partly restored the peptidylarginine deiminase 1 defect caused by IL-22. Collectively, we show that IL-22 downregulates deimination, thus identifying a potential target for treatment of skin barrier defects.

KW - Acitretin/pharmacology

KW - Biopsy

KW - Cell Differentiation/drug effects

KW - Cell Line

KW - Citrullination/drug effects

KW - Dermatitis, Atopic/drug therapy

KW - Down-Regulation

KW - Epidermis/drug effects

KW - Filaggrin Proteins/metabolism

KW - Humans

KW - Interleukins/metabolism

KW - Keratin-1/metabolism

KW - Keratinocytes/drug effects

KW - Primary Cell Culture

KW - Protein-Arginine Deiminase Type 1/genetics

KW - Psoriasis/drug therapy

KW - Vitamin D/pharmacology

U2 - 10.1016/j.jid.2021.07.155

DO - 10.1016/j.jid.2021.07.155

M3 - Journal article

C2 - 34352263

VL - 142

SP - 333-342.e6

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

IS - 2

ER -

ID: 312711148