Human thymic epithelial cells express functional HLA-DP molecules

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Human thymic epithelial cells express functional HLA-DP molecules. / Jørgensen, A; Röpke, C; Nielsen, M; Madsen, H; Svejgaard, A; Odum, Niels.

In: HLA, Vol. 47, No. 4, 1996, p. 300-6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jørgensen, A, Röpke, C, Nielsen, M, Madsen, H, Svejgaard, A & Odum, N 1996, 'Human thymic epithelial cells express functional HLA-DP molecules', HLA, vol. 47, no. 4, pp. 300-6.

APA

Jørgensen, A., Röpke, C., Nielsen, M., Madsen, H., Svejgaard, A., & Odum, N. (1996). Human thymic epithelial cells express functional HLA-DP molecules. HLA, 47(4), 300-6.

Vancouver

Jørgensen A, Röpke C, Nielsen M, Madsen H, Svejgaard A, Odum N. Human thymic epithelial cells express functional HLA-DP molecules. HLA. 1996;47(4):300-6.

Author

Jørgensen, A ; Röpke, C ; Nielsen, M ; Madsen, H ; Svejgaard, A ; Odum, Niels. / Human thymic epithelial cells express functional HLA-DP molecules. In: HLA. 1996 ; Vol. 47, No. 4. pp. 300-6.

Bibtex

@article{90e88e90fd9411ddb219000ea68e967b,
title = "Human thymic epithelial cells express functional HLA-DP molecules",
abstract = "HLA-DP molecules function as restriction elements in the presentation of foreign antigens to T cells by antigen presenting cells and certain HLA-DP molecules confer susceptibility to autoimmune disease. Because HLA molecules play an essential role in thymic selection and elimination of autoreactive T lymphocytes, we examined whether human thymic epithelial cells (TEC) expressed HLA-DP molecules. We present evidence that TEC obtained from short time culture express low but significant levels of HLA-DP molecules. The expression of HLA-DP molecules was comparable to or higher than the expression of HLA-DQ but lower than that of HLA-DR. Upon IFN-gamma treatment, HLA-DP expression was strongly upregulated. Since HLA-DQ and DR expression was upregulated in parallel, the hierarchy between MHC class II isotypes remained unchanged following interferon treatment. TEC elicited significant proliferation of HLA-DP allospecific primed lymphocyte typing (PLT) CD4 T cell lines. IFN-gamma treatment strongly upregulated the HLA-DP allospecific PLT responses whereas other PLT responses remained largely unchanged. In conclusion, these data indicate that human thymus epithelial cells express significant levels of functional HLA-DP molecules.",
author = "A J{\o}rgensen and C R{\"o}pke and M Nielsen and H Madsen and A Svejgaard and Niels Odum",
note = "Keywords: Child, Preschool; Epithelial Cells; Epithelium; HLA-DP Antigens; Humans; Infant; Interferon-gamma; Thymus Gland",
year = "1996",
language = "English",
volume = "47",
pages = "300--6",
journal = "HLA",
issn = "2059-2302",
publisher = "Wiley",
number = "4",

}

RIS

TY - JOUR

T1 - Human thymic epithelial cells express functional HLA-DP molecules

AU - Jørgensen, A

AU - Röpke, C

AU - Nielsen, M

AU - Madsen, H

AU - Svejgaard, A

AU - Odum, Niels

N1 - Keywords: Child, Preschool; Epithelial Cells; Epithelium; HLA-DP Antigens; Humans; Infant; Interferon-gamma; Thymus Gland

PY - 1996

Y1 - 1996

N2 - HLA-DP molecules function as restriction elements in the presentation of foreign antigens to T cells by antigen presenting cells and certain HLA-DP molecules confer susceptibility to autoimmune disease. Because HLA molecules play an essential role in thymic selection and elimination of autoreactive T lymphocytes, we examined whether human thymic epithelial cells (TEC) expressed HLA-DP molecules. We present evidence that TEC obtained from short time culture express low but significant levels of HLA-DP molecules. The expression of HLA-DP molecules was comparable to or higher than the expression of HLA-DQ but lower than that of HLA-DR. Upon IFN-gamma treatment, HLA-DP expression was strongly upregulated. Since HLA-DQ and DR expression was upregulated in parallel, the hierarchy between MHC class II isotypes remained unchanged following interferon treatment. TEC elicited significant proliferation of HLA-DP allospecific primed lymphocyte typing (PLT) CD4 T cell lines. IFN-gamma treatment strongly upregulated the HLA-DP allospecific PLT responses whereas other PLT responses remained largely unchanged. In conclusion, these data indicate that human thymus epithelial cells express significant levels of functional HLA-DP molecules.

AB - HLA-DP molecules function as restriction elements in the presentation of foreign antigens to T cells by antigen presenting cells and certain HLA-DP molecules confer susceptibility to autoimmune disease. Because HLA molecules play an essential role in thymic selection and elimination of autoreactive T lymphocytes, we examined whether human thymic epithelial cells (TEC) expressed HLA-DP molecules. We present evidence that TEC obtained from short time culture express low but significant levels of HLA-DP molecules. The expression of HLA-DP molecules was comparable to or higher than the expression of HLA-DQ but lower than that of HLA-DR. Upon IFN-gamma treatment, HLA-DP expression was strongly upregulated. Since HLA-DQ and DR expression was upregulated in parallel, the hierarchy between MHC class II isotypes remained unchanged following interferon treatment. TEC elicited significant proliferation of HLA-DP allospecific primed lymphocyte typing (PLT) CD4 T cell lines. IFN-gamma treatment strongly upregulated the HLA-DP allospecific PLT responses whereas other PLT responses remained largely unchanged. In conclusion, these data indicate that human thymus epithelial cells express significant levels of functional HLA-DP molecules.

M3 - Journal article

C2 - 8773319

VL - 47

SP - 300

EP - 306

JO - HLA

JF - HLA

SN - 2059-2302

IS - 4

ER -

ID: 10635915