FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis
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FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis. / Ryder, L Rebekka; Bartels, Else Marie; Woetmann, Anders; Madsen, Hans Ole; Ødum, Niels; Bliddal, Henning; Danneskiold-Samsøe, Bente; Ribel-Madsen, Søren; Ryder, Lars P.
In: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Vol. 120, No. 5, 05.2012, p. 387-396.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis
AU - Ryder, L Rebekka
AU - Bartels, Else Marie
AU - Woetmann, Anders
AU - Madsen, Hans Ole
AU - Ødum, Niels
AU - Bliddal, Henning
AU - Danneskiold-Samsøe, Bente
AU - Ribel-Madsen, Søren
AU - Ryder, Lars P
N1 - © 2011 The Authors. APMIS © 2011 APMIS.
PY - 2012/5
Y1 - 2012/5
N2 - Our aim was to elucidate the relative amount of the different splice forms of FoxP3 mRNA in CD4+ T cells in peripheral blood (PB) compared to synovial fluid (SF) in RA and PsA patients. FoxP3 mRNA was measured using a quantitative real-time PCR method. CD4+ T cells were isolated from 17 paired samples of PB and SF from RA and PsA patients, and PB from 10 controls. FoxP3fl and FoxP3Δ2 mRNA was significantly increased (6.7 and 2.1-fold, respectively) in PB CD4+ T cells from RA patients compared to controls. FoxP3fl and Δ2 mRNA in SF CD4+ T cells was increased compared to controls in sero-negative RA and PsA, but not in sero-positive RA patients, who had a high FoxP3 expression in both PB and SF. The FoxP3Δ2Δ7 mRNA was barely detectable in patient samples, and not at all in healthy individuals. We provide evidence of an increased expression of FoxP3 splice forms in synovial CD4+ T cells from RA patients. A skewed, high expression profile of FoxP3, but not CTLA-4, in sero-negative RA and PsA, indicates that synovial CD4+ T cells may represent unique subsets of T cells which have been induced locally or selectively recruited to the joint.
AB - Our aim was to elucidate the relative amount of the different splice forms of FoxP3 mRNA in CD4+ T cells in peripheral blood (PB) compared to synovial fluid (SF) in RA and PsA patients. FoxP3 mRNA was measured using a quantitative real-time PCR method. CD4+ T cells were isolated from 17 paired samples of PB and SF from RA and PsA patients, and PB from 10 controls. FoxP3fl and FoxP3Δ2 mRNA was significantly increased (6.7 and 2.1-fold, respectively) in PB CD4+ T cells from RA patients compared to controls. FoxP3fl and Δ2 mRNA in SF CD4+ T cells was increased compared to controls in sero-negative RA and PsA, but not in sero-positive RA patients, who had a high FoxP3 expression in both PB and SF. The FoxP3Δ2Δ7 mRNA was barely detectable in patient samples, and not at all in healthy individuals. We provide evidence of an increased expression of FoxP3 splice forms in synovial CD4+ T cells from RA patients. A skewed, high expression profile of FoxP3, but not CTLA-4, in sero-negative RA and PsA, indicates that synovial CD4+ T cells may represent unique subsets of T cells which have been induced locally or selectively recruited to the joint.
KW - Adult
KW - Aged
KW - Aged, 80 and over
KW - Alternative Splicing
KW - Arthritis, Psoriatic
KW - Arthritis, Rheumatoid
KW - CD4-Positive T-Lymphocytes
KW - Female
KW - Forkhead Transcription Factors
KW - Humans
KW - Male
KW - Middle Aged
KW - Protein Isoforms
KW - RNA, Messenger
KW - Real-Time Polymerase Chain Reaction
KW - Statistics, Nonparametric
KW - Synovial Fluid
KW - Young Adult
U2 - 10.1111/j.1600-0463.2011.02848.x
DO - 10.1111/j.1600-0463.2011.02848.x
M3 - Journal article
C2 - 22515293
VL - 120
SP - 387
EP - 396
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 5
ER -
ID: 46486743