Expression of full-length and splice forms of FoxP3 in rheumatoid arthritis
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Expression of full-length and splice forms of FoxP3 in rheumatoid arthritis. / Ryder, L R; Woetmann, A; Madsen, H O; Ødum, N; Ryder, L P; Bliddal, H; Danneskiold-Samsøe, B; Ribel-Madsen, S.; Bartels, E M; Ryder, Lisa Rebekka; Danielsen, Alex Le Woetmann; Madsen, H O; Ødum, Niels; Ryder, Lars P.; Bliddal, H; Danneskiold-Samsøe, B; Ribel-Madsen, S; Bartels, E M.
In: Scandinavian Journal of Rheumatology, Vol. 39, No. 4, 01.08.2010, p. 279-86.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Expression of full-length and splice forms of FoxP3 in rheumatoid arthritis
AU - Ryder, L R
AU - Woetmann, A
AU - Madsen, H O
AU - Ødum, N
AU - Ryder, L P
AU - Bliddal, H
AU - Danneskiold-Samsøe, B
AU - Ribel-Madsen, S.
AU - Bartels, E M
AU - Ryder, Lisa Rebekka
AU - Danielsen, Alex Le Woetmann
AU - Madsen, H O
AU - Ødum, Niels
AU - Ryder, Lars P.
AU - Bliddal, H
AU - Danneskiold-Samsøe, B
AU - Ribel-Madsen, S
AU - Bartels, E M
N1 - Keywords: Adult; Aged; Aged, 80 and over; Arthritis, Rheumatoid; CD4-Positive T-Lymphocytes; Female; Flow Cytometry; Forkhead Transcription Factors; Humans; Male; Middle Aged; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction
PY - 2010/8/1
Y1 - 2010/8/1
N2 - OBJECTIVE: The aim of our study was to compare the presence of full-length and alternative splice forms of FoxP3 mRNA in CD4 cells from rheumatoid arthritis (RA) patients and healthy controls. METHODS: A quantitative real-time polymerase chain reaction (QRT-PCR) method was used to measure the amount of FoxP3 mRNA full-length and splice forms. CD4-positive T cells were isolated from peripheral blood from 50 RA patients by immunomagnetic separation, and the FoxP3 mRNA expression was compared with the results from 10 healthy controls. RESULTS: We observed an increased expression of full-length FoxP3 mRNA in RA patients when compared to healthy controls, as well as an increase in CD25 mRNA expression, but no corresponding increase in CTLA-4 mRNA expression. The presence of an alternative splice form of FoxP3 lacking exon 2 was confirmed in both RA patients and healthy controls, but with no significant difference in expression between the two groups. There was a positive correlation between the amount of FoxP3 mRNA and the clinical inflammation parameters C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR), and a negative correlation between FoxP3 mRNA and the dose of methotrexate (MTX) given to the patients. CONCLUSION: RA patients express more full-length FoxP3 than healthy controls in peripheral blood CD4-positive cells, suggesting an increased number of regulatory T cells (Tregs). However, no concomitant increase in CTLA-4 expression was seen. We therefore propose that the Tregs are left unable to suppress the ongoing inflammation due to a deficiency in CTLA-4 needed for cell contact-dependent suppression.
AB - OBJECTIVE: The aim of our study was to compare the presence of full-length and alternative splice forms of FoxP3 mRNA in CD4 cells from rheumatoid arthritis (RA) patients and healthy controls. METHODS: A quantitative real-time polymerase chain reaction (QRT-PCR) method was used to measure the amount of FoxP3 mRNA full-length and splice forms. CD4-positive T cells were isolated from peripheral blood from 50 RA patients by immunomagnetic separation, and the FoxP3 mRNA expression was compared with the results from 10 healthy controls. RESULTS: We observed an increased expression of full-length FoxP3 mRNA in RA patients when compared to healthy controls, as well as an increase in CD25 mRNA expression, but no corresponding increase in CTLA-4 mRNA expression. The presence of an alternative splice form of FoxP3 lacking exon 2 was confirmed in both RA patients and healthy controls, but with no significant difference in expression between the two groups. There was a positive correlation between the amount of FoxP3 mRNA and the clinical inflammation parameters C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR), and a negative correlation between FoxP3 mRNA and the dose of methotrexate (MTX) given to the patients. CONCLUSION: RA patients express more full-length FoxP3 than healthy controls in peripheral blood CD4-positive cells, suggesting an increased number of regulatory T cells (Tregs). However, no concomitant increase in CTLA-4 expression was seen. We therefore propose that the Tregs are left unable to suppress the ongoing inflammation due to a deficiency in CTLA-4 needed for cell contact-dependent suppression.
U2 - 10.3109/03009740903555374
DO - 10.3109/03009740903555374
M3 - Journal article
C2 - 20476861
VL - 39
SP - 279
EP - 286
JO - Scandinavian Journal of Rheumatology
JF - Scandinavian Journal of Rheumatology
SN - 0300-9742
IS - 4
ER -
ID: 22127168