Enhanced sensitization and elicitation responses caused by mixtures of common fragrance allergens
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Enhanced sensitization and elicitation responses caused by mixtures of common fragrance allergens. / Bonefeld, Charlotte Menné; Nielsen, Morten Milek; Rubin, Ingrid Maria Cecilia; Vennegaard, Marie Torp; Dabelsteen, Sally; Gimenéz-Arnau, Elena; Lepoittevin, Jean-Pierre; Geisler, Carsten; Johansen, Jeanne Duus.
In: Contact Dermatitis, Vol. 65, No. 6, 12.2011, p. 336-342.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Enhanced sensitization and elicitation responses caused by mixtures of common fragrance allergens
AU - Bonefeld, Charlotte Menné
AU - Nielsen, Morten Milek
AU - Rubin, Ingrid Maria Cecilia
AU - Vennegaard, Marie Torp
AU - Dabelsteen, Sally
AU - Gimenéz-Arnau, Elena
AU - Lepoittevin, Jean-Pierre
AU - Geisler, Carsten
AU - Johansen, Jeanne Duus
N1 - © 2011 John Wiley & Sons A/S.
PY - 2011/12
Y1 - 2011/12
N2 - Background. Perfumes are complex mixtures composed of many fragrance ingredients, many of which are known to be only weak allergens when tested individually. It is therefore surprising that fragrance contact allergy is one of the most common forms of contact allergy. Objectives. To investigate whether mixing different fragrance allergens leads to increased sensitization potency, and to examine the difference in the challenge response to one chemical in mice sensitized either with the mixture of allergens or with only the relevant allergen. Methods. CBA mice were sensitized with three different concentrations of three fragrance allergens alone or as a mixture. The sensitization and elicitation responses were measured by ear thickness plus infiltration of B and T cells and T cell proliferation in the draining lymph nodes. Results. We found a dose-dependent sensitization response for each of the allergens. An increased response was seen when the allergens were mixed. A stronger challenge response to cinnamal was seen in mice sensitized with the allergen mixture than in mice sensitized with cinnamal alone. Conclusions. Our findings suggest that mixtures of allergens increase the primary response that potentiates the generation of memory T cells in response to the specific allergen. Thus, allergen mixtures enhance both induction and elicitation of contact allergy.
AB - Background. Perfumes are complex mixtures composed of many fragrance ingredients, many of which are known to be only weak allergens when tested individually. It is therefore surprising that fragrance contact allergy is one of the most common forms of contact allergy. Objectives. To investigate whether mixing different fragrance allergens leads to increased sensitization potency, and to examine the difference in the challenge response to one chemical in mice sensitized either with the mixture of allergens or with only the relevant allergen. Methods. CBA mice were sensitized with three different concentrations of three fragrance allergens alone or as a mixture. The sensitization and elicitation responses were measured by ear thickness plus infiltration of B and T cells and T cell proliferation in the draining lymph nodes. Results. We found a dose-dependent sensitization response for each of the allergens. An increased response was seen when the allergens were mixed. A stronger challenge response to cinnamal was seen in mice sensitized with the allergen mixture than in mice sensitized with cinnamal alone. Conclusions. Our findings suggest that mixtures of allergens increase the primary response that potentiates the generation of memory T cells in response to the specific allergen. Thus, allergen mixtures enhance both induction and elicitation of contact allergy.
KW - Acrolein
KW - Aldehydes
KW - Allergens
KW - Animals
KW - CD4 Lymphocyte Count
KW - CD8-Positive T-Lymphocytes
KW - Cell Proliferation
KW - Cells, Cultured
KW - Cyclohexenes
KW - Dermatitis, Allergic Contact
KW - Dose-Response Relationship, Immunologic
KW - Eugenol
KW - Female
KW - Flow Cytometry
KW - Mice
KW - Mice, Inbred CBA
KW - Perfume
U2 - 10.1111/j.1600-0536.2011.01945.x
DO - 10.1111/j.1600-0536.2011.01945.x
M3 - Journal article
C2 - 21767274
VL - 65
SP - 336
EP - 342
JO - Contact Dermatitis
JF - Contact Dermatitis
SN - 0105-1873
IS - 6
ER -
ID: 38170387