TY - JOUR

T1 - Diagnostic microRNA profiling in cutaneous T-cell lymphoma (CTCL)

AU - Ralfkiaer, Ulrik

AU - Hagedorn, Peter

AU - Bangsgaard, Nannie

AU - Løvendorf, Marianne B

AU - Ahler, Charlotte B

AU - Svensson, Lars

AU - Kopp, Katharina L

AU - Vennegaard, Marie T

AU - Lauenborg, Britt

AU - Zibert, John R

AU - Krejsgaard, Thorbjørn

AU - Bonefeld, Charlotte M

AU - Søkilde, Rolf

AU - Gjerdrum, Lise M

AU - Labuda, Tord

AU - Mathiesen, Anne-Merete

AU - Grønbaek, Kirsten

AU - Wasik, Mariusz A

AU - Sokolowska-Wojdylo, Malgorzata

AU - Queille-Roussel, Catherine

AU - Gniadecki, Robert

AU - Ralfkiaer, Elisabeth

AU - Geisler, Carsten

AU - Litman, Thomas

AU - Andersen, Anders Woetmann

AU - Glue, Christian

AU - Røpke, Mads A

AU - Skov, Lone

AU - Ødum, Niels

PY - 2011

Y1 - 2011

N2 - Cutaneous T-cell lymphomas (CTCLs) are the most frequent primary skin lymphomas. Nevertheless, diagnosis of early disease has proven difficult because of a clinical and histologic resemblance to benign inflammatory skin diseases. To address whether microRNA (miRNA) profiling can discriminate CTCL from benign inflammation, we studied miRNA expression levels in 198 patients with CTCL, peripheral T-cell lymphoma (PTL), and benign skin diseases (psoriasis and dermatitis). Using microarrays, we show that the most induced (miR-326, miR-663b, and miR-711) and repressed (miR-203 and miR-205) miRNAs distinguish CTCL from benign skin diseases with > 90% accuracy in a training set of 90 samples and a test set of 58 blinded samples. These miRNAs also distinguish malignant and benign lesions in an independent set of 50 patients with PTL and skin inflammation and in experimental human xenograft mouse models of psoriasis and CTCL. Quantitative (q)RT-PCR analysis of 103 patients with CTCL and benign skin disorders validates differential expression of 4 of the 5 miRNAs and confirms previous reports on miR-155 in CTCL. A qRT-PCR-based classifier consisting of miR-155, miR-203, and miR-205 distinguishes CTCL from benign disorders with high specificity and sensitivity, and with a classification accuracy of 95%, indicating that miRNAs have a high diagnostic potential in CTCL.

AB - Cutaneous T-cell lymphomas (CTCLs) are the most frequent primary skin lymphomas. Nevertheless, diagnosis of early disease has proven difficult because of a clinical and histologic resemblance to benign inflammatory skin diseases. To address whether microRNA (miRNA) profiling can discriminate CTCL from benign inflammation, we studied miRNA expression levels in 198 patients with CTCL, peripheral T-cell lymphoma (PTL), and benign skin diseases (psoriasis and dermatitis). Using microarrays, we show that the most induced (miR-326, miR-663b, and miR-711) and repressed (miR-203 and miR-205) miRNAs distinguish CTCL from benign skin diseases with > 90% accuracy in a training set of 90 samples and a test set of 58 blinded samples. These miRNAs also distinguish malignant and benign lesions in an independent set of 50 patients with PTL and skin inflammation and in experimental human xenograft mouse models of psoriasis and CTCL. Quantitative (q)RT-PCR analysis of 103 patients with CTCL and benign skin disorders validates differential expression of 4 of the 5 miRNAs and confirms previous reports on miR-155 in CTCL. A qRT-PCR-based classifier consisting of miR-155, miR-203, and miR-205 distinguishes CTCL from benign disorders with high specificity and sensitivity, and with a classification accuracy of 95%, indicating that miRNAs have a high diagnostic potential in CTCL.

U2 - 10.1182/blood-2011-06-358382

DO - 10.1182/blood-2011-06-358382

M3 - Journal article

C2 - 21865341

VL - 118

SP - 5891

EP - 5900

JO - Blood

JF - Blood

SN - 0006-4971

IS - 22

ER -