Characterization of T cell receptor assembly and expression in a Ti gamma delta-positive cell line

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Standard

Characterization of T cell receptor assembly and expression in a Ti gamma delta-positive cell line. / Kuhlmann, J; Caspar-Bauguil, S; Geisler, C; Rubin, B.

In: European Journal of Immunology, Vol. 23, No. 2, 1993, p. 487-93.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kuhlmann, J, Caspar-Bauguil, S, Geisler, C & Rubin, B 1993, 'Characterization of T cell receptor assembly and expression in a Ti gamma delta-positive cell line', European Journal of Immunology, vol. 23, no. 2, pp. 487-93.

APA

Kuhlmann, J., Caspar-Bauguil, S., Geisler, C., & Rubin, B. (1993). Characterization of T cell receptor assembly and expression in a Ti gamma delta-positive cell line. European Journal of Immunology, 23(2), 487-93.

Vancouver

Kuhlmann J, Caspar-Bauguil S, Geisler C, Rubin B. Characterization of T cell receptor assembly and expression in a Ti gamma delta-positive cell line. European Journal of Immunology. 1993;23(2):487-93.

Author

Kuhlmann, J ; Caspar-Bauguil, S ; Geisler, C ; Rubin, B. / Characterization of T cell receptor assembly and expression in a Ti gamma delta-positive cell line. In: European Journal of Immunology. 1993 ; Vol. 23, No. 2. pp. 487-93.

Bibtex

@article{33781e40b0a711ddb538000ea68e967b,
title = "Characterization of T cell receptor assembly and expression in a Ti gamma delta-positive cell line",
abstract = "T cell antigen receptor (TcR) heterodimers of both the Ti-alpha beta and Ti-gamma delta types are expressed at the surface of T cells noncovalently associated with the CD3 complex composed of the monomorphic chains gamma, delta, epsilon and zeta. The structural relationship and assembly of the various components of this multimeric protein complex is still not fully understood. In this report, the human leukemic T cell line Lyon which expresses a Ti-gamma delta/CD3 complex, was characterized and compared to another human leukemic T cell line Jurkat (Ti-alpha beta/CD3). Membrane TCR-/CD3- variants of the T cell Lyon were induced and found to produce all of the Ti/CD3 components, with the exception of Ti-delta. Biochemical analysis indicated that: (1) Ti-gamma/CD3 gamma, delta, epsilon complexes were formed in the endoplasmic reticulum in the absence of Ti-delta; (2) the CD3-zeta chain did not associate with the Ti-gamma/CD3 gamma delta epsilon complex and (3) the Ti-delta chain was required for cell surface expression of the Ti-gamma delta/CD3 complex. Introduction of Jurkat wild-type Ti-alpha cDNA into Lyon T cells resulted in Ti-alpha beta/CD3 expression and abrogated Ti-gamma delta/CD3 expression. In contrast, the expression of the Ti-gamma delta/CD3 complex was not affected by transfection of a mutated Ti-alpha cDNA into Lyon cells. The mutated Ti-alpha chain formed complexes with Ti-beta and CD3 gamma delta epsilon, but the CD3-zeta chain did not associate with these complexes. Taken together analysis of Lyon cells transfected with either wild-type or mutated Ti-alpha suggested that the CD3-zeta chain may have higher affinity for Ti-alpha beta/CD3 complexes than for Ti-gamma delta/CD3 complexes.",
author = "J Kuhlmann and S Caspar-Bauguil and C Geisler and B Rubin",
note = "Keywords: Biological Transport; Blotting, Northern; Blotting, Southern; Cell Line; Endoplasmic Reticulum; Flow Cytometry; Humans; Receptor-CD3 Complex, Antigen, T-Cell; Receptors, Antigen, T-Cell; Receptors, Antigen, T-Cell, alpha-beta; Receptors, Antigen, T-Cell, gamma-delta; T-Lymphocytes; Transfection; Tumor Cells, Cultured",
year = "1993",
language = "English",
volume = "23",
pages = "487--93",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "2",

}

RIS

TY - JOUR

T1 - Characterization of T cell receptor assembly and expression in a Ti gamma delta-positive cell line

AU - Kuhlmann, J

AU - Caspar-Bauguil, S

AU - Geisler, C

AU - Rubin, B

N1 - Keywords: Biological Transport; Blotting, Northern; Blotting, Southern; Cell Line; Endoplasmic Reticulum; Flow Cytometry; Humans; Receptor-CD3 Complex, Antigen, T-Cell; Receptors, Antigen, T-Cell; Receptors, Antigen, T-Cell, alpha-beta; Receptors, Antigen, T-Cell, gamma-delta; T-Lymphocytes; Transfection; Tumor Cells, Cultured

PY - 1993

Y1 - 1993

N2 - T cell antigen receptor (TcR) heterodimers of both the Ti-alpha beta and Ti-gamma delta types are expressed at the surface of T cells noncovalently associated with the CD3 complex composed of the monomorphic chains gamma, delta, epsilon and zeta. The structural relationship and assembly of the various components of this multimeric protein complex is still not fully understood. In this report, the human leukemic T cell line Lyon which expresses a Ti-gamma delta/CD3 complex, was characterized and compared to another human leukemic T cell line Jurkat (Ti-alpha beta/CD3). Membrane TCR-/CD3- variants of the T cell Lyon were induced and found to produce all of the Ti/CD3 components, with the exception of Ti-delta. Biochemical analysis indicated that: (1) Ti-gamma/CD3 gamma, delta, epsilon complexes were formed in the endoplasmic reticulum in the absence of Ti-delta; (2) the CD3-zeta chain did not associate with the Ti-gamma/CD3 gamma delta epsilon complex and (3) the Ti-delta chain was required for cell surface expression of the Ti-gamma delta/CD3 complex. Introduction of Jurkat wild-type Ti-alpha cDNA into Lyon T cells resulted in Ti-alpha beta/CD3 expression and abrogated Ti-gamma delta/CD3 expression. In contrast, the expression of the Ti-gamma delta/CD3 complex was not affected by transfection of a mutated Ti-alpha cDNA into Lyon cells. The mutated Ti-alpha chain formed complexes with Ti-beta and CD3 gamma delta epsilon, but the CD3-zeta chain did not associate with these complexes. Taken together analysis of Lyon cells transfected with either wild-type or mutated Ti-alpha suggested that the CD3-zeta chain may have higher affinity for Ti-alpha beta/CD3 complexes than for Ti-gamma delta/CD3 complexes.

AB - T cell antigen receptor (TcR) heterodimers of both the Ti-alpha beta and Ti-gamma delta types are expressed at the surface of T cells noncovalently associated with the CD3 complex composed of the monomorphic chains gamma, delta, epsilon and zeta. The structural relationship and assembly of the various components of this multimeric protein complex is still not fully understood. In this report, the human leukemic T cell line Lyon which expresses a Ti-gamma delta/CD3 complex, was characterized and compared to another human leukemic T cell line Jurkat (Ti-alpha beta/CD3). Membrane TCR-/CD3- variants of the T cell Lyon were induced and found to produce all of the Ti/CD3 components, with the exception of Ti-delta. Biochemical analysis indicated that: (1) Ti-gamma/CD3 gamma, delta, epsilon complexes were formed in the endoplasmic reticulum in the absence of Ti-delta; (2) the CD3-zeta chain did not associate with the Ti-gamma/CD3 gamma delta epsilon complex and (3) the Ti-delta chain was required for cell surface expression of the Ti-gamma delta/CD3 complex. Introduction of Jurkat wild-type Ti-alpha cDNA into Lyon T cells resulted in Ti-alpha beta/CD3 expression and abrogated Ti-gamma delta/CD3 expression. In contrast, the expression of the Ti-gamma delta/CD3 complex was not affected by transfection of a mutated Ti-alpha cDNA into Lyon cells. The mutated Ti-alpha chain formed complexes with Ti-beta and CD3 gamma delta epsilon, but the CD3-zeta chain did not associate with these complexes. Taken together analysis of Lyon cells transfected with either wild-type or mutated Ti-alpha suggested that the CD3-zeta chain may have higher affinity for Ti-alpha beta/CD3 complexes than for Ti-gamma delta/CD3 complexes.

M3 - Journal article

C2 - 8436182

VL - 23

SP - 487

EP - 493

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 2

ER -

ID: 8546083