A novel xenograft model of cutaneous T-cell lymphoma

Research output: Contribution to journal › Journal article › Research › peer-review

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A novel xenograft model of cutaneous T-cell lymphoma. / Krejsgaard, Thorbjørn; Kopp, Katharina; Ralfkiaer, Elisabeth; Willumsgaard, Ayelah E; Eriksen, Karsten W; Labuda, Tord; Rasmussen, Susanne; Mathiesen, Anne-Merete; Geisler, Carsten; Lauenborg, Britt; Becker, Jürgen C; Zhang, Qian; Wasik, Mariusz A; Odum, Niels; Woetmann, Anders.

In: Experimental Dermatology, Vol. 19, No. 12, 2010, p. 1096-102.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Krejsgaard, T, Kopp, K, Ralfkiaer, E, Willumsgaard, AE, Eriksen, KW, Labuda, T, Rasmussen, S, Mathiesen, A-M, Geisler, C, Lauenborg, B, Becker, JC, Zhang, Q, Wasik, MA, Odum, N & Woetmann, A 2010, 'A novel xenograft model of cutaneous T-cell lymphoma', Experimental Dermatology, vol. 19, no. 12, pp. 1096-102. https://doi.org/10.1111/j.1600-0625.2010.01138.x

APA

Krejsgaard, T., Kopp, K., Ralfkiaer, E., Willumsgaard, A. E., Eriksen, K. W., Labuda, T., Rasmussen, S., Mathiesen, A-M., Geisler, C., Lauenborg, B., Becker, J. C., Zhang, Q., Wasik, M. A., Odum, N., & Woetmann, A. (2010). A novel xenograft model of cutaneous T-cell lymphoma. Experimental Dermatology, 19(12), 1096-102. https://doi.org/10.1111/j.1600-0625.2010.01138.x

Vancouver

Krejsgaard T, Kopp K, Ralfkiaer E, Willumsgaard AE, Eriksen KW, Labuda T et al. A novel xenograft model of cutaneous T-cell lymphoma. Experimental Dermatology. 2010;19(12):1096-102. https://doi.org/10.1111/j.1600-0625.2010.01138.x

Author

Krejsgaard, Thorbjørn ; Kopp, Katharina ; Ralfkiaer, Elisabeth ; Willumsgaard, Ayelah E ; Eriksen, Karsten W ; Labuda, Tord ; Rasmussen, Susanne ; Mathiesen, Anne-Merete ; Geisler, Carsten ; Lauenborg, Britt ; Becker, Jürgen C ; Zhang, Qian ; Wasik, Mariusz A ; Odum, Niels ; Woetmann, Anders. / A novel xenograft model of cutaneous T-cell lymphoma. In: Experimental Dermatology. 2010 ; Vol. 19, No. 12. pp. 1096-102.

Bibtex

@article{b5eed490c55e11df825b000ea68e967b,
title = "A novel xenograft model of cutaneous T-cell lymphoma",
abstract = "Cutaneous T-cell lymphomas (CTCLs) are characterized by accumulation of malignant T cells in the skin. Early disease resembles benign skin disorders but during disease progression cutaneous tumors develop, and eventually the malignant T cells can spread to lymph nodes and internal organs. However, because of the lack of suitable animal models, little is known about the mechanisms driving CTCL development and progression in vivo. Here, we describe a novel xenograft model of tumor stage CTCL, where malignant T cells (MyLa2059) are transplanted to NOD/SCID-B2m(-/-) (NOD.Cg-Prkdc(scid) B2m(tm1Unc)/J) mice. Subcutaneous transplantation of the malignant T cells led to rapid tumor formation in 43 of 48 transplantations, whereas transplantation of non-malignant T cells isolated from the same donor did not result in tumor development. Importantly, the tumor growth was significantly suppressed in mice treated with vorinostat when compared to mice treated with vehicle. Furthermore, in most mice the tumors displayed subcutaneous and/or lymphatic dissemination. Histological, immunohistochemical and flow cytometric analyses confirmed that both tumors at the inoculation site, as well as distant subcutaneous and lymphatic tumors, originated from the transplanted malignant T cells. In conclusion, we describe a novel mouse model of tumor stage CTCL for future studies of disease dissemination and preclinical evaluations of new therapeutic strategies.",
author = "Thorbj{\o}rn Krejsgaard and Katharina Kopp and Elisabeth Ralfkiaer and Willumsgaard, {Ayelah E} and Eriksen, {Karsten W} and Tord Labuda and Susanne Rasmussen and Anne-Merete Mathiesen and Carsten Geisler and Britt Lauenborg and Becker, {J{\"u}rgen C} and Qian Zhang and Wasik, {Mariusz A} and Niels Odum and Anders Woetmann",
note = "Keywords:cutaneous T-cell lymphoma;metastasis;mouse model;mycosis fungoides;vorinostat",
year = "2010",
doi = "10.1111/j.1600-0625.2010.01138.x",
language = "English",
volume = "19",
pages = "1096--102",
journal = "Experimental Dermatology",
issn = "0906-6705",
publisher = "Wiley-Blackwell",
number = "12",

}

RIS

TY - JOUR

T1 - A novel xenograft model of cutaneous T-cell lymphoma

AU - Krejsgaard, Thorbjørn

AU - Kopp, Katharina

AU - Ralfkiaer, Elisabeth

AU - Willumsgaard, Ayelah E

AU - Eriksen, Karsten W

AU - Labuda, Tord

AU - Rasmussen, Susanne

AU - Mathiesen, Anne-Merete

AU - Geisler, Carsten

AU - Lauenborg, Britt

AU - Becker, Jürgen C

AU - Zhang, Qian

AU - Wasik, Mariusz A

AU - Odum, Niels

AU - Woetmann, Anders

N1 - Keywords:cutaneous T-cell lymphoma;metastasis;mouse model;mycosis fungoides;vorinostat

PY - 2010

Y1 - 2010

N2 - Cutaneous T-cell lymphomas (CTCLs) are characterized by accumulation of malignant T cells in the skin. Early disease resembles benign skin disorders but during disease progression cutaneous tumors develop, and eventually the malignant T cells can spread to lymph nodes and internal organs. However, because of the lack of suitable animal models, little is known about the mechanisms driving CTCL development and progression in vivo. Here, we describe a novel xenograft model of tumor stage CTCL, where malignant T cells (MyLa2059) are transplanted to NOD/SCID-B2m(-/-) (NOD.Cg-Prkdc(scid) B2m(tm1Unc)/J) mice. Subcutaneous transplantation of the malignant T cells led to rapid tumor formation in 43 of 48 transplantations, whereas transplantation of non-malignant T cells isolated from the same donor did not result in tumor development. Importantly, the tumor growth was significantly suppressed in mice treated with vorinostat when compared to mice treated with vehicle. Furthermore, in most mice the tumors displayed subcutaneous and/or lymphatic dissemination. Histological, immunohistochemical and flow cytometric analyses confirmed that both tumors at the inoculation site, as well as distant subcutaneous and lymphatic tumors, originated from the transplanted malignant T cells. In conclusion, we describe a novel mouse model of tumor stage CTCL for future studies of disease dissemination and preclinical evaluations of new therapeutic strategies.

AB - Cutaneous T-cell lymphomas (CTCLs) are characterized by accumulation of malignant T cells in the skin. Early disease resembles benign skin disorders but during disease progression cutaneous tumors develop, and eventually the malignant T cells can spread to lymph nodes and internal organs. However, because of the lack of suitable animal models, little is known about the mechanisms driving CTCL development and progression in vivo. Here, we describe a novel xenograft model of tumor stage CTCL, where malignant T cells (MyLa2059) are transplanted to NOD/SCID-B2m(-/-) (NOD.Cg-Prkdc(scid) B2m(tm1Unc)/J) mice. Subcutaneous transplantation of the malignant T cells led to rapid tumor formation in 43 of 48 transplantations, whereas transplantation of non-malignant T cells isolated from the same donor did not result in tumor development. Importantly, the tumor growth was significantly suppressed in mice treated with vorinostat when compared to mice treated with vehicle. Furthermore, in most mice the tumors displayed subcutaneous and/or lymphatic dissemination. Histological, immunohistochemical and flow cytometric analyses confirmed that both tumors at the inoculation site, as well as distant subcutaneous and lymphatic tumors, originated from the transplanted malignant T cells. In conclusion, we describe a novel mouse model of tumor stage CTCL for future studies of disease dissemination and preclinical evaluations of new therapeutic strategies.

U2 - 10.1111/j.1600-0625.2010.01138.x

DO - 10.1111/j.1600-0625.2010.01138.x

M3 - Journal article

C2 - 20629733

VL - 19

SP - 1096

EP - 1102

JO - Experimental Dermatology

JF - Experimental Dermatology

SN - 0906-6705

IS - 12

ER -

ID: 22127033