Seminar by Senior Researcher Brian Tate Weinert, DTU

Seminar title: High-throughput high-efficiency immune cell engineering for immune therapy and functional analysis.

The LEO Foundation Skin Immunology Research Center is pleased to welcome Senior Researcher Brian Tate Weinert from the Novo Nordisk Foundation Center for Biosustainability, DTU.

In the Cell Architecture Laboratory, Brian Tate Weiner develops CRISPR/Cas12a-based methods for high-throughput (96-well) and high-efficiency genome engineering. Using pre-assembled ribonucleoprotein (RNP) complexes, they can achieve high rates of transgene insertion (>25%), gene knock-out (>90%), and gene reprogramming (>40%). These methods are applied to build chimeric antigen receptor (CAR) T-cells using CRISPR for precise CAR insertion with homogenous expression levels and concurrent knock-out of checkpoint proteins such as PD1. The lab also develops methods to investigate sequence-activity relationships by multiplexed gene reprogramming and transgene library insertion followed by functional selection. These methods provide a powerful platform to build next-generation cellular immune therapies and to create custom cell models for discovery in basic research.

Time and place: 4 March 2021, 14:30-16:00, https://ucph-ku.zoom.us/j/66045523220