Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression. / Geisler, C.

In: Journal of Immunology, Vol. 148, No. 8, 1992, p. 2437-45.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Geisler, C 1992, 'Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression', Journal of Immunology, vol. 148, no. 8, pp. 2437-45.

APA

Geisler, C. (1992). Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression. Journal of Immunology, 148(8), 2437-45.

Vancouver

Geisler C. Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression. Journal of Immunology. 1992;148(8):2437-45.

Author

Geisler, C. / Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression. In: Journal of Immunology. 1992 ; Vol. 148, No. 8. pp. 2437-45.

Bibtex

@article{ecac3a40b0a711ddb538000ea68e967b,
title = "Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression",
abstract = "The TCR consists of the Ti alpha beta heterodimer and the associated CD3 chains, CD3 gamma delta epsilon zeta 2 or zeta eta. The structural relationships between the subunits of the Ti/CD3 complex are still not fully understood. To explore the roles of the individual CD3 chains for the assembly, intracellular processing, and expression of the TCR, mutants of the T cell line Jurkat were isolated. One variant, JGN, was found to produce all the Ti/CD3 components with the exception of CD3-gamma. The results indicate that: 1) the tetrameric form (Ti alpha beta-CD3 delta epsilon) of the Ti/CD3 complex is produced in the endoplasmic reticulum in the absence of CD3-gamma; 2) CD3-zeta does not associate with the Ti alpha beta-CD3 delta epsilon complex; 3) the Ti alpha beta-CD3 delta epsilon complex is not exported from the endoplasmic reticulum to the Golgi apparatus; and 4) CD3-gamma is required for cell surface expression of the Ti/CD3 complex. Transfection of the wild-type CD3-gamma gene into JGN reconstituted expression of functional Ti/CD3 complexes, and analysis of T cell lines producing different amounts of CD3-gamma indicated that CD3-gamma and CD3-delta competed for the binding to CD3-epsilon.",
author = "C Geisler",
note = "Keywords: Antigens, CD3; Antigens, Differentiation, T-Lymphocyte; Base Sequence; Cell Line; DNA; Humans; Molecular Sequence Data; Receptors, Antigen, T-Cell; T-Lymphocytes; Transfection",
year = "1992",
language = "English",
volume = "148",
pages = "2437--45",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "8",

}

RIS

TY - JOUR

T1 - Failure to synthesize the CD3-gamma chain. Consequences for T cell antigen receptor assembly, processing, and expression

AU - Geisler, C

N1 - Keywords: Antigens, CD3; Antigens, Differentiation, T-Lymphocyte; Base Sequence; Cell Line; DNA; Humans; Molecular Sequence Data; Receptors, Antigen, T-Cell; T-Lymphocytes; Transfection

PY - 1992

Y1 - 1992

N2 - The TCR consists of the Ti alpha beta heterodimer and the associated CD3 chains, CD3 gamma delta epsilon zeta 2 or zeta eta. The structural relationships between the subunits of the Ti/CD3 complex are still not fully understood. To explore the roles of the individual CD3 chains for the assembly, intracellular processing, and expression of the TCR, mutants of the T cell line Jurkat were isolated. One variant, JGN, was found to produce all the Ti/CD3 components with the exception of CD3-gamma. The results indicate that: 1) the tetrameric form (Ti alpha beta-CD3 delta epsilon) of the Ti/CD3 complex is produced in the endoplasmic reticulum in the absence of CD3-gamma; 2) CD3-zeta does not associate with the Ti alpha beta-CD3 delta epsilon complex; 3) the Ti alpha beta-CD3 delta epsilon complex is not exported from the endoplasmic reticulum to the Golgi apparatus; and 4) CD3-gamma is required for cell surface expression of the Ti/CD3 complex. Transfection of the wild-type CD3-gamma gene into JGN reconstituted expression of functional Ti/CD3 complexes, and analysis of T cell lines producing different amounts of CD3-gamma indicated that CD3-gamma and CD3-delta competed for the binding to CD3-epsilon.

AB - The TCR consists of the Ti alpha beta heterodimer and the associated CD3 chains, CD3 gamma delta epsilon zeta 2 or zeta eta. The structural relationships between the subunits of the Ti/CD3 complex are still not fully understood. To explore the roles of the individual CD3 chains for the assembly, intracellular processing, and expression of the TCR, mutants of the T cell line Jurkat were isolated. One variant, JGN, was found to produce all the Ti/CD3 components with the exception of CD3-gamma. The results indicate that: 1) the tetrameric form (Ti alpha beta-CD3 delta epsilon) of the Ti/CD3 complex is produced in the endoplasmic reticulum in the absence of CD3-gamma; 2) CD3-zeta does not associate with the Ti alpha beta-CD3 delta epsilon complex; 3) the Ti alpha beta-CD3 delta epsilon complex is not exported from the endoplasmic reticulum to the Golgi apparatus; and 4) CD3-gamma is required for cell surface expression of the Ti/CD3 complex. Transfection of the wild-type CD3-gamma gene into JGN reconstituted expression of functional Ti/CD3 complexes, and analysis of T cell lines producing different amounts of CD3-gamma indicated that CD3-gamma and CD3-delta competed for the binding to CD3-epsilon.

M3 - Journal article

C2 - 1532815

VL - 148

SP - 2437

EP - 2445

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 8

ER -

ID: 8546216